Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development

The mygalomorph spider Aphonopelma hentzi (Girard, 1852) (Texas brown tarantula) is a geographically widespread and accessible system for the study of comparative development in spiders. However, remarkably little information is available regarding the timing of egg sac deposition and duration of em...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of arachnology 2019-05, Vol.47 (2), p.209-216
Main Authors: Setton, Emily V.W., Hendrixson, Brent E., Sharma, Prashant P.
Format: Article
Language:English
Subjects:
Embryonic development
FEATURED ARTICLES
Grasslands
Immunohistochemistry
Ontogeny
opisthosoma
Parasteatoda tepidariorum
spider
spinnerets
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3
cites cdi_FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3
container_end_page 216
container_issue 2
container_start_page 209
container_title The Journal of arachnology
container_volume 47
creator Setton, Emily V.W.
Hendrixson, Brent E.
Sharma, Prashant P.
description The mygalomorph spider Aphonopelma hentzi (Girard, 1852) (Texas brown tarantula) is a geographically widespread and accessible system for the study of comparative development in spiders. However, remarkably little information is available regarding the timing of egg sac deposition and duration of embryogenesis in this species, a gap that can directly affect the success of field collecting efforts. Here, we describe the habitat, egg sacs, and embryos of A. hentzi in the Comanche National Grasslands of Colorado. We compare the effectiveness of strategies for obtaining adult specimens vs. egg sacs of A. hentzi from burrows. Protocols for rearing, fixation, fluorescent immunohistochemistry, colorimetric in situ hybridization, and imaging of embryos are detailed. Together with forthcoming genomic resources, these data are anticipated to improve understanding of embryogenesis in Mygalomorphae and comparative development of chelicerates more generally.
doi_str_mv 10.1636/JoA-S-18-081
format article
fullrecord <record><control><sourceid>gale_cross</sourceid><recordid>TN_cdi_gale_infotracmisc_A605611323</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A605611323</galeid><jstor_id>26872310</jstor_id><sourcerecordid>A605611323</sourcerecordid><originalsourceid>FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3</originalsourceid><addsrcrecordid>eNp9kU9r3DAQxU1podu0t14Lgl6yIU71J5a9uZklTVNSCt30LMbSeK1gW0bShrrfLd-tWra0LJSigzSa33vzYLLsLaMXTAr54bOr803OqpxW7Fm2YCtR5lys5PNsQZlkecUpf5m9CuGBpprTcpE9XQ-Nn90WRww2EDsSIGvXOw_GkclNux6idSNxLamnzo1uwn4A0uEYf1pyemM9eHNOWFXwJTmtPYwIeEW-zFvo3eD81O3L-w49JHmwBnB5RTBEaHobOjtu08DJu8GG_TvMIeJAWudJ7JCEuDPzfvbw148YfMTeTUOK8Dp70UIf8M3v-yT7_vH6fv0pv_t6c7uu7_JGrETMmwaM4WWpC9NWl1CusEAjUHDUxaVGrMBILlEwoQXXnJWgTQUgqWhLWZhGnGTvD74pBSo7ti560CmzVrWkhWRMcJGoi39Q6RgcrHYjtjb9HwmWR4LERPwRt7ALQd1uvh2z5wdWexeCx1ZN3g7gZ8Wo2m9fpe2rjWKVSttP-LsD_hCi839YLquSC0ZT_-zQb6xLyf5v9gs_Srxy</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development</title><source>JSTOR Archival Journals and Primary Sources Collection</source><creator>Setton, Emily V.W. ; Hendrixson, Brent E. ; Sharma, Prashant P.</creator><creatorcontrib>Setton, Emily V.W. ; Hendrixson, Brent E. ; Sharma, Prashant P.</creatorcontrib><description>The mygalomorph spider Aphonopelma hentzi (Girard, 1852) (Texas brown tarantula) is a geographically widespread and accessible system for the study of comparative development in spiders. However, remarkably little information is available regarding the timing of egg sac deposition and duration of embryogenesis in this species, a gap that can directly affect the success of field collecting efforts. Here, we describe the habitat, egg sacs, and embryos of A. hentzi in the Comanche National Grasslands of Colorado. We compare the effectiveness of strategies for obtaining adult specimens vs. egg sacs of A. hentzi from burrows. Protocols for rearing, fixation, fluorescent immunohistochemistry, colorimetric in situ hybridization, and imaging of embryos are detailed. Together with forthcoming genomic resources, these data are anticipated to improve understanding of embryogenesis in Mygalomorphae and comparative development of chelicerates more generally.</description><identifier>ISSN: 0161-8202</identifier><identifier>EISSN: 1937-2396</identifier><identifier>DOI: 10.1636/JoA-S-18-081</identifier><language>eng</language><publisher>American Arachnological Society</publisher><subject>Embryonic development ; FEATURED ARTICLES ; Grasslands ; Immunohistochemistry ; Ontogeny ; opisthosoma ; Parasteatoda tepidariorum ; spider ; spinnerets</subject><ispartof>The Journal of arachnology, 2019-05, Vol.47 (2), p.209-216</ispartof><rights>COPYRIGHT 2019 The American Arachnological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3</citedby><cites>FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26872310$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26872310$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,58213,58446</link.rule.ids></links><search><creatorcontrib>Setton, Emily V.W.</creatorcontrib><creatorcontrib>Hendrixson, Brent E.</creatorcontrib><creatorcontrib>Sharma, Prashant P.</creatorcontrib><title>Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development</title><title>The Journal of arachnology</title><description>The mygalomorph spider Aphonopelma hentzi (Girard, 1852) (Texas brown tarantula) is a geographically widespread and accessible system for the study of comparative development in spiders. However, remarkably little information is available regarding the timing of egg sac deposition and duration of embryogenesis in this species, a gap that can directly affect the success of field collecting efforts. Here, we describe the habitat, egg sacs, and embryos of A. hentzi in the Comanche National Grasslands of Colorado. We compare the effectiveness of strategies for obtaining adult specimens vs. egg sacs of A. hentzi from burrows. Protocols for rearing, fixation, fluorescent immunohistochemistry, colorimetric in situ hybridization, and imaging of embryos are detailed. Together with forthcoming genomic resources, these data are anticipated to improve understanding of embryogenesis in Mygalomorphae and comparative development of chelicerates more generally.</description><subject>Embryonic development</subject><subject>FEATURED ARTICLES</subject><subject>Grasslands</subject><subject>Immunohistochemistry</subject><subject>Ontogeny</subject><subject>opisthosoma</subject><subject>Parasteatoda tepidariorum</subject><subject>spider</subject><subject>spinnerets</subject><issn>0161-8202</issn><issn>1937-2396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU9r3DAQxU1podu0t14Lgl6yIU71J5a9uZklTVNSCt30LMbSeK1gW0bShrrfLd-tWra0LJSigzSa33vzYLLsLaMXTAr54bOr803OqpxW7Fm2YCtR5lys5PNsQZlkecUpf5m9CuGBpprTcpE9XQ-Nn90WRww2EDsSIGvXOw_GkclNux6idSNxLamnzo1uwn4A0uEYf1pyemM9eHNOWFXwJTmtPYwIeEW-zFvo3eD81O3L-w49JHmwBnB5RTBEaHobOjtu08DJu8GG_TvMIeJAWudJ7JCEuDPzfvbw148YfMTeTUOK8Dp70UIf8M3v-yT7_vH6fv0pv_t6c7uu7_JGrETMmwaM4WWpC9NWl1CusEAjUHDUxaVGrMBILlEwoQXXnJWgTQUgqWhLWZhGnGTvD74pBSo7ti560CmzVrWkhWRMcJGoi39Q6RgcrHYjtjb9HwmWR4LERPwRt7ALQd1uvh2z5wdWexeCx1ZN3g7gZ8Wo2m9fpe2rjWKVSttP-LsD_hCi839YLquSC0ZT_-zQb6xLyf5v9gs_Srxy</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Setton, Emily V.W.</creator><creator>Hendrixson, Brent E.</creator><creator>Sharma, Prashant P.</creator><general>American Arachnological Society</general><general>The American Arachnological Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope></search><sort><creationdate>20190501</creationdate><title>Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development</title><author>Setton, Emily V.W. ; Hendrixson, Brent E. ; Sharma, Prashant P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Embryonic development</topic><topic>FEATURED ARTICLES</topic><topic>Grasslands</topic><topic>Immunohistochemistry</topic><topic>Ontogeny</topic><topic>opisthosoma</topic><topic>Parasteatoda tepidariorum</topic><topic>spider</topic><topic>spinnerets</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Setton, Emily V.W.</creatorcontrib><creatorcontrib>Hendrixson, Brent E.</creatorcontrib><creatorcontrib>Sharma, Prashant P.</creatorcontrib><collection>CrossRef</collection><collection>Science in Context</collection><jtitle>The Journal of arachnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Setton, Emily V.W.</au><au>Hendrixson, Brent E.</au><au>Sharma, Prashant P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development</atitle><jtitle>The Journal of arachnology</jtitle><date>2019-05-01</date><risdate>2019</risdate><volume>47</volume><issue>2</issue><spage>209</spage><epage>216</epage><pages>209-216</pages><issn>0161-8202</issn><eissn>1937-2396</eissn><abstract>The mygalomorph spider Aphonopelma hentzi (Girard, 1852) (Texas brown tarantula) is a geographically widespread and accessible system for the study of comparative development in spiders. However, remarkably little information is available regarding the timing of egg sac deposition and duration of embryogenesis in this species, a gap that can directly affect the success of field collecting efforts. Here, we describe the habitat, egg sacs, and embryos of A. hentzi in the Comanche National Grasslands of Colorado. We compare the effectiveness of strategies for obtaining adult specimens vs. egg sacs of A. hentzi from burrows. Protocols for rearing, fixation, fluorescent immunohistochemistry, colorimetric in situ hybridization, and imaging of embryos are detailed. Together with forthcoming genomic resources, these data are anticipated to improve understanding of embryogenesis in Mygalomorphae and comparative development of chelicerates more generally.</abstract><pub>American Arachnological Society</pub><doi>10.1636/JoA-S-18-081</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0161-8202
ispartof The Journal of arachnology, 2019-05, Vol.47 (2), p.209-216
issn 0161-8202
1937-2396
language eng
recordid cdi_gale_infotracmisc_A605611323
source JSTOR Archival Journals and Primary Sources Collection
subjects Embryonic development
FEATURED ARTICLES
Grasslands
Immunohistochemistry
Ontogeny
opisthosoma
Parasteatoda tepidariorum
spider
spinnerets
title Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T15%3A40%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Embryogenesis%20in%20a%20Colorado%20population%20of%20Aphonopelma%20hentzi%20(Girard,%201852)%20(Araneae:%20Mygalomorphae:%20Theraphosidae):%20establishing%20a%20promising%20system%20for%20the%20study%20of%20mygalomorph%20development&rft.jtitle=The%20Journal%20of%20arachnology&rft.au=Setton,%20Emily%20V.W.&rft.date=2019-05-01&rft.volume=47&rft.issue=2&rft.spage=209&rft.epage=216&rft.pages=209-216&rft.issn=0161-8202&rft.eissn=1937-2396&rft_id=info:doi/10.1636/JoA-S-18-081&rft_dat=%3Cgale_cross%3EA605611323%3C/gale_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b393t-bbadd277c5df84a79e5ed3e32ec54cee8ad626e313c32c217acd8aa603f765db3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A605611323&rft_jstor_id=26872310&rfr_iscdi=true