HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1[alpha]

Background: Breast cancer is the most common malignant tumor that affects women with higher incidence. High-mobility group box 3 (HMGB3) plays critical functions in DNA repair, recombination, transcription and replication. This study aimed to investigate the effects of HMGB3 silence on mammosphere f...

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Bibliographic Details
Published in:Cancer management and research 2019-05, p.5075
Main Authors: Gu, Jun, Xu, Tao, Huang, Qin-Hua, Zhang, Chu-Miao, Chen, Hai-Yan
Format: Article
Language:English
Subjects:
Analysis
Anopheles
Biological markers
Breast cancer
Cancer cells
Chromosomal proteins
DNA
DNA repair
Genetic research
Growth
Luciferase
Silence
Transcription (Genetics)
Tumors
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Summary:Background: Breast cancer is the most common malignant tumor that affects women with higher incidence. High-mobility group box 3 (HMGB3) plays critical functions in DNA repair, recombination, transcription and replication. This study aimed to investigate the effects of HMGB3 silence on mammosphere formation and tumor growth of breast cancer. Methods: LV5-HMGB3 and LV3-siHMGB3 vectors were transfected into MCF10A, MDA-MB-231, HCC1937, ZR-75-1 and MCF7 cells. Cell counting kit-8 (CCK-8) assay was used to evaluate cell proliferation. Xenograft tumor mice model was established by injection of MDA-MB-231. qRT-PCR and western blot were used to examine the expression of Nanog, Sox2 and OCT-4. Mammosphere forming assay was employed to evaluate mammosphere formation both in vivo and in vitro. Dual luciferase assay was utilized to verify the interaction between HMGB3 and hypoxia-inducible factor 1[alpha] (HIF1[alpha]). CD[44.sup.+]/CD[24.sup.-] was assessed with flow cytometry. Results: HMGB3 expression was higher significantly (p
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S204357