Ageing-related changes in the levels of [beta]-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7 in the heart of men

Aging is a major risk factor for morbidity and mortality from cardiovascular causes in men. To better understand the cellular processes related to age-related cardiac complications, we undertook research aimed at comparative evaluation of genes expression and distribution of [beta]-catenin, CacyBP/S...

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Bibliographic Details
Published in:PloS one 2020-03, Vol.15 (3), p.e0229462
Main Authors: Kasacka, Irena, Piotrowska, Zaneta, Niezgoda, Michal, Lewandowska, Alicja, Lebkowski, Wojciech
Format: Article
Language:English
Subjects:
Angiotensin II
Biochemistry
Cardiovascular diseases
Diseases
Gene expression
Genes
Homeostasis
Morbidity
Organ transplantation
Time
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Summary:Aging is a major risk factor for morbidity and mortality from cardiovascular causes in men. To better understand the cellular processes related to age-related cardiac complications, we undertook research aimed at comparative evaluation of genes expression and distribution of [beta]-catenin, CacyBP/SIP, galectin-3 and LMP7 in the heart of healthy men in different age groups. The study was conducted on the hearts of 12 men (organ donors) without a history of cardiovascular disease, who were divided into two age groups: men under and men over 45 years of age. On paraffin sections, immunohistochemical reactions were performed to detect [beta]-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7. The expression of genes coding [beta]-catenin, CacyBP/SIP, galectin-3 and LMP7 was also evaluated by real-time PCR method. In the heart of men over 45 years old, both gene expression and immunoreactivity of [beta]-catenin, CacyBP/SIP, galectin-3 and LMP7 were stronger compared to younger individuals. The results of the presented studies suggest that [beta]-catenin, CacyBP/SIP, galectin-3 and immunoproteasomes might be involved in the internal regulation of heart homeostasis during ageing.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0229462