Ammonium Salts Promote Functional Adaptation of Rat Erythrocytes on the Model of Forced Swimming

• Published in: Bulletin of experimental biology and medicine 2020-02, Vol.168 (4), p.444-448
• Main Authors: Novozhilov, A. V., Mindukshev, I. V., Korf, E. A., Krivchenko, A. I., Goncharov, N. V.
• Format: Article
• Language: English
• Subjects: 2,3-Diphosphoglycerate - agonists; 2,3-Diphosphoglycerate - metabolism; 5'-Nucleotidase - genetics; 5'-Nucleotidase - metabolism; Adaptation, Physiological - drug effects; Adaptation, Physiological - physiology; Adenosine triphosphatase; Amino acids; Ammonium Chloride - pharmacology; Ammonium compounds; Ammonium paratungstate; Animals; Antioxidants - pharmacology; Biomedical and Life Sciences; Biomedicine; Calcium-Transporting ATPases - genetics; Calcium-Transporting ATPases - metabolism; Carbonates; Carbonates - pharmacology; Cell Biology; Comparative analysis; Dose-Response Relationship, Drug; Erythrocytes - cytology; Erythrocytes - drug effects; Erythrocytes - metabolism; Gene Expression - drug effects; Glutathione - agonists; Glutathione - metabolism; Internal Medicine; Laboratory Medicine; Nucleotidases; Oxidative Stress - drug effects; Pathology; Physical Conditioning, Animal; Physical Exertion; Rats; Sodium-Potassium-Exchanging ATPase - genetics; Sodium-Potassium-Exchanging ATPase - metabolism; Swimming
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-020-04728-6

Ammonium, an end-product of catabolism, in low doses can promote adaptation of metabolic pathways in erythrocytes under conditions of extreme physical exercise. We compared the effects of two ammonium salts, ammonium chloride and ammonium carbonate, in two doses on biochemical parameters of rat erythrocytes 1 day after extreme physical exercise in a 4-week cycle of forced swimming. Of 16 analyzed parameters, the maximum number of significant shifts from the control was revealed in the groups of rats receiving ammonium chloride in doses of 20 and 10 mg/kg, and the minimal number of differences was found in groups treated with ammonium carbonate in the same doses. The comparison of the levels of reduced glutathione and 2.3-bisphosphoglicerate and activities of 5’-nucleotidase and Ca 2+ - and Na/K-ATPases attested to more rigorous control of the mechanism of oxygen delivery to tissues by erythrocytes after administration of ammonium chloride in a dose of 20 mg/kg.