The Overexpression of T[beta]4 in the Hair Follicle Tissue of Alpas Cashmere Goats Increases Cashmere Yield and Promotes Hair Follicle Development

• Published in: Animals (Basel) 2020-01, Vol.10 (1)
• Main Authors: Dai, Bai, Liang, Hao, Guo, Dong-dong, Bi, Zhao-wei, Yuan, Jian-long, Jin, Yong, Huan, Lei, Guo, Xu-dong, Cang, Ming, Liu, Dong-jun
• Format: Article
• Language: English
• Subjects: Cashmere; Genetic aspects; Genetically modified animals; Goats; Hair follicles; Physiological aspects; Zoological research
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani10010075

Cashmere goats have double coats consisting of non-modulated fine inner hairs or cashmere fibers produced by secondary hair follicles (SHFs) and guard hairs produced by primary hair follicles (PHFs). Cashmere is an important economic product worldwide. The world market for cashmere is increasing while the current production of cashmere is limited. Thymosin beta-4 (T[beta]4), a 4.9 kDa protein, contains 43 amino acids. Here, we investigated whether T[beta]4 overexpression would increase SHF numbers, and thus improve the cashmere yield. We produced T[beta]4 transgenic goats using a tissue-specific overexpression strategy. The T[beta]4 overexpression (T[beta]4-OE) goats had increased hair follicle development and higher cashmere yields than the wild type (WT) goats. The development of this goat model is not only valuable as a framework for future studies of the mechanism of goat follicular development, but will also lead to the improvement of economically important cashmere traits. Increased cashmere yield and improved quality are some goals of cashmere goat breeding. Thymosin beta-4 (T[beta]4) plays a key role in the growth and development of hair follicles. For the past ten years, we have evaluated the role of T[beta]4 by establishing a flock of 15 cashmere goats that specifically overexpress the T[beta]4 gene in the hair follicles. These T[beta]4 overexpression (T[beta]4-OE) cashmere goats had more secondary hair follicles than the WT goats and produced more cashmere. Meanwhile, combined analysis of the skin transcriptome and proteome in cashmere goats suggested that T[beta]4 may affect hair growth by interacting with keratin type II cytoskeletal 4 epidermal (KRT4) to mediate the extracellular signal-regulated protein kinase (ERK) signaling pathway, thereby promoting the development of secondary hair follicles, and consequently, increasing cashmere yield. Thus, the specific overexpression of T[beta]4 in the hair follicles of cashmere goats effectively increased the cashmere yield. Keywords: T[beta]4; secondary hair follicle; cashmere goat; SCNT