Benzopyrene exposure induced neuronal loss, plaque deposition, and cognitive decline in APP/PS1 mice

• Published in: Journal of neuroinflammation 2020-08, Vol.17 (1)
• Main Authors: Liu, Dan, Zhao, Yujia, Qi, Yuze, Gao, Yun, Tu, Dezhen, Wang, Yinxi, Gao, Hui-Ming, Zhou, Hui
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Benzopyrene; Brain research; Care and treatment; Development and progression; Health aspects
• ISSN: 1742-2094; 1742-2094
• DOI: 10.1186/s12974-020-01925-y

Background Exposure to benzo(a)pyrene (BaP) was associated with cognitive impairments and some Alzheimer's disease (AD)-like pathological changes. However, it is largely unknown whether BaP exposure participates in the disease progression of AD. Objectives To investigate the effect of BaP exposure on AD progression and its underlying mechanisms. Methods BaP or vehicle was administered to 4-month-old APPswe/PS1dE9 transgenic (APP/PS1) mice and wildtype (WT) mice for 2 months. Learning and memory ability and exploratory behaviors were evaluated 1 month after the initiation/termination of BaP exposure. AD-like pathological and biochemical alterations were examined 1 month after 2-month BaP exposure. Levels of soluble beta-amyloid (A[beta]) oligomers and the number of A[beta] plaques in the cortex and the hippocampus were quantified. Gene expression profiling was used to evaluate alternation of genes/pathways associated with AD onset and progression. Immunohistochemistry and Western blot were used to demonstrate neuronal loss and neuroinflammation in the cortex and the hippocampus. Treatment of primary neuron-glia cultures with aged A[beta] (a mixture of monomers, oligomers, and fibrils) and/or BaP was used to investigate mechanisms by which BaP enhanced A[beta]-induced neurodegeneration. Results BaP exposure induced progressive decline in spatial learning/memory and exploratory behaviors in APP/PS1 mice and WT mice, and APP/PS1 mice showed severer behavioral deficits than WT mice. Moreover, BaP exposure promoted neuronal loss, A[beta] burden and A[beta] plaque formation in APP/PS1 mice, but not in WT mice. Gene expression profiling showed most robust alteration in genes and pathways related to inflammation and immunoregulatory process, A[beta] secretion and degradation, and synaptic formation in WT and APP/PS1 mice after BaP exposure. Consistently, the cortex and the hippocampus of WT and APP/PS1 mice displayed activation of microglia and astroglia and upregulation of inducible nitric oxide synthase (iNOS), glial fibrillary acidic protein (GFAP), and NADPH oxidase (three widely used neuroinflammatory markers) after BaP exposure. Furthermore, BaP exposure aggravated neurodegeneration induced by aged A[beta] peptide in primary neuron-glia cultures through enhancing NADPH oxidase-derived oxidative stress. Conclusion Our study showed that chronic exposure to environmental pollutant BaP induced, accelerated, and exacerbated the progression of AD, in which elevated