Pinocembrin Ameliorates Cognitive Impairment Induced by Vascular Dementia: Contribution of Reelin-dabl Signaling Pathway

Background: As a substrate of apoER2, Reelin has been verified to exert neuroprotection by preventing memory impairment. Pinocembrin is the most abundant natural flavonoid found in propolis, and it has been used to exert neuroprotection, blood-brain barrier protection, anti-oxidation, and inflammati...

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Published in:Drug design, development and therapy development and therapy, 2020-09, Vol.14, p.3577
Main Authors: Kang, Ze-Chun, Wang, Hai-Gang, Yang, Yu-Lin, Zhao, Xiao-Yue, Zhou, Qi-Meng, Yang, Ying-Lin, Yang, Jing-Yu, Du, Guan-Hua
Format: Article
Language:English
Subjects:
Animal behavior
Animal experimentation
Flavonoids
Molecular biology
Neurons
Vascular dementia
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Summary:Background: As a substrate of apoER2, Reelin has been verified to exert neuroprotection by preventing memory impairment. Pinocembrin is the most abundant natural flavonoid found in propolis, and it has been used to exert neuroprotection, blood-brain barrier protection, anti-oxidation, and inflammation diminishing, both in vitro and in vivo. However, the roles and molecular mechanisms of pinocembrin in neurobehavioral outcomes and neuronal repair after vascular dementia are still under investigation. Purpose: To explore the role of pinocembrin in the involvement of the Reelin-dabl signaling pathway in improving memory impairment, both in cell culture and animals experiments. Material and Methods: Behavioral tests were conducted on day 48 to confirm the protection of pinocembrin against cognitive impairment. Cell and molecular biology experiments demonstrated that the Reelin-dabl pathway mediates the underlying mechanism of cognitive improvement by pinocembrin. Results: It was showed that pinocembrin alleviated learning and memory deficits induced by vascular dementia, by inducing the expression of Reelin, apoER2, and p-dabl in the hippocampus. The expression of Reelin and p-dabl was both inhibited following Reelin RNA interference in SH-SY5Y prior to oxygen glucose deprivation (OGD) injury, suggesting that Reelin played a core role in pinocembrin's effect on OGD in vitro. Conclusion: Pinocembrin improves the cognition via the Reelin-dabl signaling pathway. Keywords: pinocembrin, Reelin, vascular dementia, hippocampus, cognitive impairment, signaling pathway
ISSN:1177-8881
1177-8881
DOI:10.2147/DDDT.S249176