Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes/Caracterizacao quimica e atividade antimicrobiana de Campomanesia aurea contra tres cepas de Listeria monocytogenes

• Published in: Brazilian journal of biology 2021-02, Vol.81 (1), p.69
• Main Authors: Pacheco, L.A, Ethur, E.M, Sheibel, T, Buhl, B, Weber, A.C, Kauffmann, C, Marchi, M.I, Freitas, E.M, Hoehne, L
• Format: Article
• Language: English
• ISSN: 1519-6984; 1678-4375
• DOI: 10.1590/1519-6984.219889

The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in Sao Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was [alpha]-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was [alpha]-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.