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Yersinia massiliensis : primeiro relato de associacao com insetos

Endosymbiont bacteria can affect biological parameters and reduce the effectiveness of natural enemies in controlling the target insect. The objective of this work was to identify endosymbiont bacteria in Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), the main natural enemy used to manage...

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Bibliographic Details
Published in:Brazilian journal of biology 2022-02, Vol.82
Main Authors: Ribeiro, M.F, Carvalho, V.R, Favoreto, A.L, De Marchi, B.R, Jordan, C, Zanuncio, J.C, Soares, M.A, Zanuncio, A.J.V, Wilcken, C.F
Format: Article
Language:English
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Summary:Endosymbiont bacteria can affect biological parameters and reduce the effectiveness of natural enemies in controlling the target insect. The objective of this work was to identify endosymbiont bacteria in Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), the main natural enemy used to manage Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae). Genomic DNA from six A. nitens populations was extracted and polymerase chain reactions (PCR) were performed with the primers to detect endosymbiont bacteria in this insect. The PCR products were amplified, sequenced, and compared with sequences deposited in the GenBank for the bacteria identification. All A. nitens populations had the bacterium Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae). This bacterium was originally described as free-living, and it is associated with and composes part of the A. nitens microbiota. This is the first report of Y. massiliensis in an insect host. Keywords: biological control, egg parasitoid, microbiota, molecular entomology. As bacterias endossimbiontes podem afetar os parametros biologicos e reduzirem a eficacia de inimigos naturais no controle do inseto alvo. O objetivo deste trabalho foi identificar bacterias endossimbiontes em Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), o principal inimigo natural usado no manejo de Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae). O DNA genomico de seis populacoes de A. nitens foi extraido e as reacoes em cadeia da polimerase (PCR) realizadas com os primers para detectar bacterias endossimbiontes neste inseto. Os produtos de PCR foram amplificados, sequenciados e comparados com as sequencias depositadas no GenBank para identificacao das bacterias. Todas as populacoes de A. nitens tinham a bacteria Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae). Esta bacteria foi originalmente descrita como de vida livre e esta associada e compoe parte da microbiota de A. nitens. Este e o primeiro relato de Y. massiliensis em um hospedeiro. Palavras-chave: controle biologico, parasitoide de ovo, microbiota, entomologia molecular.
ISSN:1519-6984
DOI:10.1590/1519-6984.237098