Radiolabeled Complex of Antimicrobial Peptides UBI29-41 and UBI18-35 Labeled with [sup.99m]Tc for Differential Diagnosis of Bone Infection of the Limbs

We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI1835, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide1-yl 6-(bis(pyridin-2-y...

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Published in:Bulletin of experimental biology and medicine 2021-02, Vol.170 (4), p.415
Main Authors: Zavadovskaya, V.D, Larkina, M.S, Stasyuk, E.S, Zorkaltsev, M.A, Udodov, V.D, Ivanov, V.V, Shevtsova, N.M, Rogov, A.S, Lushchyk, A.Ya, Yantsevich, A.V, Belousov, M.V, Baikov, A.N, Skuridin, V.S, Pershina, A.G
Format: Article
Language:English
Subjects:
Foot diseases
Health aspects
Infection
Inflammation
Peptides
SPECT imaging
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Summary:We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI1835, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes [sup.99m]TcDPAH-UBK9-41 and [sup.99m]Tc-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography. Key Words: ubiquicidin; technetium-99m; osteomyelitis; SPECT
ISSN:0007-4888
DOI:10.1007/s10517-021-05078-7