Staphylococcal cassette chromosome mec elements from Staphylococcus intermedins group de caes em um centro veterinario de diagnosticos no Brasil

• Published in: Ciência rural 2021-09, Vol.51 (9), p.1
• Main Authors: Ruiz, Regina, Barbosa, Valcilaine Teixeira, Lobo, Teresa De Lisieux Guedes Ferreira, Silva, Rafael Aleixo Dos Santos, Calumby, Rodrigo Jose Nunes, Vieira, Suzanne Pinheiro, Brandao, Diogo, Zorzi, Felipe Mendes, Moreira, Rossana Teotonio De Farias, Alvino, Valter, Nascimento, Ticiano Gomes Do, Grilo, Luciano Aparecido Meireles, Dornelas, Camila Braga, Mamizuka, Elsa Masae, Almeida, Lara Mendes De
• Format: Article
• Language: English
• ISSN: 0103-8478
• DOI: 10.1590/0103-8478cr20200936

Methicillin resistance in the Staphylococcus intermedins group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2') responsible for [beta]-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC [greater than or equal to] 0.5 [micro]g/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in Sao Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid[R] M.I. C.Evaluator[R] strips. PBP 2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates. Key words: Staphylococcus intermedius group (SIG), methicillin resistance, SCCmec elements, companion animals. A resistência à meticilina no grupo Staphylococcus intermedius (GSI) tem aumentado na clínica de pequenos animais. Membros GSI resistentes à meticilina (GSIRM) têm sido causas de infecções tanto em animais de companhia e humanos. Cassetes cromossômicos estafilocócicos mec (SCCmec) carregam os genes mecA/C, que codificam a transpeptidase PBP2a (PBP2') responsável pela resistência aos antibióticos [beta]-lactâmicos em estafilococos. Nosso objetivo foi investigar os elementos SCCmec de GSIRM isolados de diferentes amostras clínicas de cães que exibiram CIM de meticilina [mayor que o igual a] 0,5 [micrón]g/mL por meio de um sistema automatizado em um Centro Veterinário de Diagnósticos em São Paulo, Brasil. A sensibilidade à meticilina foi determinada por meio do teste de microdiluição em caldo e fitas Oxoid[R] M.I.CEvaluator[R]. A produção de PBP2a foi detectada usando um ensaio de aglutinação de látex. A ti