Immunohistochemical Analysis of 1.25-dihydroxyvitamin D3 Receptor Expression in Endometrial Cancer/Endometrium Kanserinde 1,25-dihidroksivitamin D3 Reseptor Ekspresyonunun Immunohistokimyasal Analizi

• Published in: Bagcilar Medical Bulletin 2021-06, Vol.6 (2), p.142
• Main Authors: Cetin, Nesibe Kahraman, Turan, Ozgur Deniz
• Format: Article
• Language: English
• Subjects: Alfacalcidol; Calcifediol; Comparative analysis; Endometrial cancer; Estrogen; Vitamin D
• ISSN: 2547-9431; 2547-9431
• DOI: 10.4274/BMB.galenos.2021.01.01

Objective: This study aimed to immunohistochemically investigate the expression of 1.25-dihydroxyvitamin-D3-receptors (VDR) in benign and malignant lesions of the endometrial tissue. Method: The cases were divided into two groups as benign (n = 10) and endometrioid adenocarcinoma (n = 17) according to their endometrial pathology results. The expression of VDR was examined by immunohistochemical method in endometrial tissues of participants (n = 27). Then, VDR expression levels were compared between the groups. The intensity of expression, extent of staining and overall scores were determined for the semi-quantitative evaluation of VDR expression. Demographic data of the patients were also recorded. Results: There was no significant difference between the groups in terms of demographic data (p > 0.05) except age (p < 0.001). VDR expression level in the malignant group was significantly higher than in the benign group (p < 0.001). It was observed that when the tumor grade increased, the expression level of VDR also increased significantly (r = 0.888, p < 0.001). Similarly, VDR expression increased significantly as the stage progressed (r = 0.639, p = 0.006) but there was no statistically significant correlation between tumor size (p = 0.645), estrogen receptor positivity, PR positivity and presence of lymphovascular invasion on VDR expression (p > 0.05). Conclusion: The expression of VDR was increased in endometrial cancer when compared to normal endometrial tissue. As the tumor behavior became aggressive, VDR expression also increased. As a result, increased VDR level may be associated with endometrial cancer development and stage of disease. Keywords: Endometrial cancer, vitamin D, vitamin D receptor Amac: Bu calisma, endometrial dokunun benign ve malign lezyonlarinda 1,25-dihidroksi-vitamin-D3-reseptorlerinin (VDR) ekspresyonunu immunohistokimyasal olarak arastirmak icin tasarlandi. Yontem: Olgular endometrial patoloji sonuclarina gore benign (n = 10) ve endometrioid adenokarsinom (n = 17) olarak iki gruba ayrildi. Olgulara (n = 27) ait olan endometrial dokulardaki VDR ekspresyonu immunohistokimyasal yontemlerle incelendi ve gruplar arasinda VDR ekspresyon seviyeleri karsilastirildi. VDR ekspresyonunun yari nicel degerlendirilmesi icin ekspresyon yogunlugu, boyanma dansitesi ve genel skorlar belirlendi. Hastalara ait demografik veriler kaydedildi. Bulgular: Gruplar arasinda, yas disindaki (p < 0,001) demografik verilerde anlamli fark yoktu (p > 0,05). Mal