Antimicrobial Peptides SLPI and Beta Defensin-1 in Sputum are Negatively Correlated with [FEV.sub.1]

Background: Chronic obstructive pulmonary disease (COPD) and asthma have heterogeneous inflammation with inhaled corticosteroids (ICS) as a mainstay of treatment. There is increased prevalence of non-typeable Haemophilus influenzae (NTHi) persistence in airways of patients with neutrophilic airway i...

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Bibliographic Details
Published in:International journal of chronic obstructive pulmonary disease 2021-05, Vol.16, p.1437
Main Authors: Cane, Jennifer, Tregidgo, Laura, Thulborn, Samantha, Finch, Donna, Bafadhel, Mona
Format: Article
Language:English
Subjects:
Asthma
Care and treatment
Corticosteroids
Fluticasone
Inflammation
Peptides
Protease inhibitors
Proteases
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Summary:Background: Chronic obstructive pulmonary disease (COPD) and asthma have heterogeneous inflammation with inhaled corticosteroids (ICS) as a mainstay of treatment. There is increased prevalence of non-typeable Haemophilus influenzae (NTHi) persistence in airways of patients with neutrophilic airway inflammation, potentially due to suppressed host defence after corticosteroid treatment. Antimicrobial peptides (AMPs) have antimicrobial activity against pathogens and immunomodulatory effects. We investigated whether AMPs associate with NTHi presence in COPD and asthma, and whether ICS alter this. Methods: Secretory leukocyte protease inhibitor (SLPI), osteopontin, elafin and beta defensin-1 were measured in sputum supernatants from healthy donors (n=9), asthmatics (n=21) and patients with COPD (n=14). Elafin and beta defensin-1 were measured in a primary human bronchial epithelial cells (HBECs) from healthy and COPD donors infected with NTHi and pre-treated with fluticasone propionate (FP) and budesonide (BUD). Internalised NTHi was quantified by qPCR. Results: Sputum SLPI was negatively correlated with FEV1 (p
ISSN:1178-2005
DOI:10.2147/COPD.S301622