Hepatoprotective effect of Moringa oleifera extract on TNF-[alpha] and TGF-[beta] expression in acetaminophen-induced liver fibrosis in rats

Background It has been reported that Moringa oleifera (MO) has different medicinal properties. The aim of this study was to evaluate the hepatoprotective role of Moringa oleifera extract on acetaminophen-induced liver fibrosis in albino rats on a biochemical and histological basis. Forty male albino...

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Bibliographic Details
Published in:The Egyptian journal of medical human genetics 2020-12, Vol.21 (1)
Main Authors: Aly, Omnia, Abouelfadl, Dalia M, Shaker, Olfat G, Hegazy, Gehan A, Fayez, Ahmed M, Zaki, Hanan Hassan
Format: Article
Language:English
Subjects:
Acetaminophen
Enzyme-linked immunosorbent assay
Enzymes
Fibrosis
Liver diseases
Transforming growth factors
Tumor necrosis factor
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Summary:Background It has been reported that Moringa oleifera (MO) has different medicinal properties. The aim of this study was to evaluate the hepatoprotective role of Moringa oleifera extract on acetaminophen-induced liver fibrosis in albino rats on a biochemical and histological basis. Forty male albino rats were divided into four groups: group I (control group), healthy rates; group II (acetaminophen group), rates received acetaminophen for induction of liver fibrosis; group III (treated group), liver fibrosis of rates treated with Moringa oleifera extract; and group IV (prophylactic group), rates treated with Moringa oleifera extract before and after induction of liver fibrosis. Serum liver function parameters were quantified using a spectrophotometer, while tumor necrosis factor [alpha] (TNF-[alpha]) and transformed growth factor beta (TGF- [beta]) in liver tissue homogenate by means of enzyme-linked immunosorbent assay (ELISA), and expression of liver tissue TNF-[alpha] and TGF-genes was measured by real-time PCR after extraction and purification. Hepatic tissue was also evaluated under a microscope for histopathological changes. Results Our results showed a significant decrease in liver enzymes, TNF-[alpha], and TGF-[beta] in the treated and prophylactic groups compared to the acetaminophen group, and our biochemical data were consistent with the histopathological findings confirming the hepatoprotective effect of Moringa oleifera extract. Conclusions Biochemical parameters and histopathology results provide evidence that Moringa oleifera ethanolic extract has a great potential to prevent and improve liver damage due to its protective activity.
ISSN:1110-8630
DOI:10.1186/s43042-020-00106-z