2-adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes

The present study aimed to investigate expression of [beta]2-adrenergic receptor (AR), the effect of the stress-related neurotransmitter norepinephrine (NE) on cell viability, proliferation and the therapeutic effect of propranolol, which is a typical [beta]-blocker in various type of head and neck...

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Published in:Oncology letters 2021-11, Vol.22 (5), p.1
Main Authors: Kwon, Soon Young, Chun, Kyung Ju, Jung, Narae, Shin, Hyun-Ah, Jang, Jeon Yeob, Choi, Hyo Geun, Oh, Kyoung-Ho, Kim, Min-Su
Format: Article
Language:English
Subjects:
Analysis
Aprotinin
DNA polymerases
Head and neck cancer
Health aspects
Immunohistochemistry
Propranolol hydrochloride
Scientific equipment and supplies industry
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container_issue 5
container_start_page 1
container_title Oncology letters
container_volume 22
creator Kwon, Soon Young
Chun, Kyung Ju
Jung, Narae
Shin, Hyun-Ah
Jang, Jeon Yeob
Choi, Hyo Geun
Oh, Kyoung-Ho
Kim, Min-Su
description The present study aimed to investigate expression of [beta]2-adrenergic receptor (AR), the effect of the stress-related neurotransmitter norepinephrine (NE) on cell viability, proliferation and the therapeutic effect of propranolol, which is a typical [beta]-blocker in various type of head and neck cancers for the first time. The [beta]2-AR expression was investigated using immunohistochemistry and an immunoreactive scoring (IRS) system in 57 different head and neck cancer specimens, and reverse transcriptase-polymerase chain reaction and western blotting in four head and neck cancer cell lines (HNCCLs). Cell viability and proliferation assays were performed using 0, 1, 5 and 10 [micro]M of NE and 1 [micro]M of propranolol in four HNCCLs. The expression of [beta]2-AR was positive in the majority of head and neck cancer tissues (55/57, 96.5%); however, it was signifcantly higher in oral cavity cancer than in pharyngeal cancer (median IRS: 9 vs. 3; P
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The [beta]2-AR expression was investigated using immunohistochemistry and an immunoreactive scoring (IRS) system in 57 different head and neck cancer specimens, and reverse transcriptase-polymerase chain reaction and western blotting in four head and neck cancer cell lines (HNCCLs). Cell viability and proliferation assays were performed using 0, 1, 5 and 10 [micro]M of NE and 1 [micro]M of propranolol in four HNCCLs. The expression of [beta]2-AR was positive in the majority of head and neck cancer tissues (55/57, 96.5%); however, it was signifcantly higher in oral cavity cancer than in pharyngeal cancer (median IRS: 9 vs. 3; P&lt;0.001). All HNCCLs exhibited [beta]2-AR expression, with a higher expression level detected in the oral cavity cancer cell line than in the others. NE stimulated viability (oral cavity, 206%; larynx, 156%; pharynx, 130%; nasal cavity, 137%; 10 [micro]M NE) and proliferation (124, 176, 131 and 127%, respectively) in a dose-dependent manner in all HNCCLs. Conversely, propranolol attenuated such viability (55, 42, 18 and 22%, respectively) and proliferation (22, 40, 61 and 48%, respectively). In conclusion, the viability and proliferation of various head and neck cancers may be directly stimulated by stress and this may be attenuated by [beta]-blockers.</description><identifier>ISSN: 1792-1074</identifier><identifier>DOI: 10.3892/ol.2021.13065</identifier><language>eng</language><publisher>Spandidos Publications</publisher><subject>Analysis ; Aprotinin ; DNA polymerases ; Head and neck cancer ; Health aspects ; Immunohistochemistry ; Propranolol hydrochloride ; Scientific equipment and supplies industry</subject><ispartof>Oncology letters, 2021-11, Vol.22 (5), p.1</ispartof><rights>COPYRIGHT 2021 Spandidos Publications</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Kwon, Soon Young</creatorcontrib><creatorcontrib>Chun, Kyung Ju</creatorcontrib><creatorcontrib>Jung, Narae</creatorcontrib><creatorcontrib>Shin, Hyun-Ah</creatorcontrib><creatorcontrib>Jang, Jeon Yeob</creatorcontrib><creatorcontrib>Choi, Hyo Geun</creatorcontrib><creatorcontrib>Oh, Kyoung-Ho</creatorcontrib><creatorcontrib>Kim, Min-Su</creatorcontrib><title>2-adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes</title><title>Oncology letters</title><description>The present study aimed to investigate expression of [beta]2-adrenergic receptor (AR), the effect of the stress-related neurotransmitter norepinephrine (NE) on cell viability, proliferation and the therapeutic effect of propranolol, which is a typical [beta]-blocker in various type of head and neck cancers for the first time. The [beta]2-AR expression was investigated using immunohistochemistry and an immunoreactive scoring (IRS) system in 57 different head and neck cancer specimens, and reverse transcriptase-polymerase chain reaction and western blotting in four head and neck cancer cell lines (HNCCLs). Cell viability and proliferation assays were performed using 0, 1, 5 and 10 [micro]M of NE and 1 [micro]M of propranolol in four HNCCLs. The expression of [beta]2-AR was positive in the majority of head and neck cancer tissues (55/57, 96.5%); however, it was signifcantly higher in oral cavity cancer than in pharyngeal cancer (median IRS: 9 vs. 3; P&lt;0.001). All HNCCLs exhibited [beta]2-AR expression, with a higher expression level detected in the oral cavity cancer cell line than in the others. NE stimulated viability (oral cavity, 206%; larynx, 156%; pharynx, 130%; nasal cavity, 137%; 10 [micro]M NE) and proliferation (124, 176, 131 and 127%, respectively) in a dose-dependent manner in all HNCCLs. Conversely, propranolol attenuated such viability (55, 42, 18 and 22%, respectively) and proliferation (22, 40, 61 and 48%, respectively). In conclusion, the viability and proliferation of various head and neck cancers may be directly stimulated by stress and this may be attenuated by [beta]-blockers.</description><subject>Analysis</subject><subject>Aprotinin</subject><subject>DNA polymerases</subject><subject>Head and neck cancer</subject><subject>Health aspects</subject><subject>Immunohistochemistry</subject><subject>Propranolol hydrochloride</subject><subject>Scientific equipment and supplies industry</subject><issn>1792-1074</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptUE1LAzEQzUHBUnv0HhC87ZqPbpI9luIXFLz0XrLJpBvdJkuyFcU_b1APFZyBefDmvQczCF1RUnPVsts41IwwWlNORHOGZlS2rKJELi_QIucXUqoRVCkxQ5-s0jZBgLT3BicwME4xYXgfE-TsY8A6WDz1gME5MFPG0eEQE4w-wNinMr8VY4pj0iEOccDF9KaTj8eMe9D2ex_AvGKjg4GE87GbPkbIl-jc6SHD4hfnaHt_t10_Vpvnh6f1alPtWyUrSZyxRBJFO9FwDZK2VghQkhO-7FhnnaKFdEZ11JLOdNQoKYRr9bLhTQN8jq5_Yvd6gJ0PLk5Jm4PPZrcSijHFWQmbo_ofVWkLB29iAOcL_8dwc2Iohw5Tn-NwnMrT8qnwC6EnfC4</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Kwon, Soon Young</creator><creator>Chun, Kyung Ju</creator><creator>Jung, Narae</creator><creator>Shin, Hyun-Ah</creator><creator>Jang, Jeon Yeob</creator><creator>Choi, Hyo Geun</creator><creator>Oh, Kyoung-Ho</creator><creator>Kim, Min-Su</creator><general>Spandidos Publications</general><scope/></search><sort><creationdate>20211101</creationdate><title>2-adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes</title><author>Kwon, Soon Young ; Chun, Kyung Ju ; Jung, Narae ; Shin, Hyun-Ah ; Jang, Jeon Yeob ; Choi, Hyo Geun ; Oh, Kyoung-Ho ; Kim, Min-Su</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g987-70fcd07081b653ae719d66e873034b2bdf81e71fc8b1d0bcb1c8766f9a45355e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analysis</topic><topic>Aprotinin</topic><topic>DNA polymerases</topic><topic>Head and neck cancer</topic><topic>Health aspects</topic><topic>Immunohistochemistry</topic><topic>Propranolol hydrochloride</topic><topic>Scientific equipment and supplies industry</topic><toplevel>online_resources</toplevel><creatorcontrib>Kwon, Soon Young</creatorcontrib><creatorcontrib>Chun, Kyung Ju</creatorcontrib><creatorcontrib>Jung, Narae</creatorcontrib><creatorcontrib>Shin, Hyun-Ah</creatorcontrib><creatorcontrib>Jang, Jeon Yeob</creatorcontrib><creatorcontrib>Choi, Hyo Geun</creatorcontrib><creatorcontrib>Oh, Kyoung-Ho</creatorcontrib><creatorcontrib>Kim, Min-Su</creatorcontrib><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwon, Soon Young</au><au>Chun, Kyung Ju</au><au>Jung, Narae</au><au>Shin, Hyun-Ah</au><au>Jang, Jeon Yeob</au><au>Choi, Hyo Geun</au><au>Oh, Kyoung-Ho</au><au>Kim, Min-Su</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>2-adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes</atitle><jtitle>Oncology letters</jtitle><date>2021-11-01</date><risdate>2021</risdate><volume>22</volume><issue>5</issue><spage>1</spage><pages>1-</pages><issn>1792-1074</issn><abstract>The present study aimed to investigate expression of [beta]2-adrenergic receptor (AR), the effect of the stress-related neurotransmitter norepinephrine (NE) on cell viability, proliferation and the therapeutic effect of propranolol, which is a typical [beta]-blocker in various type of head and neck cancers for the first time. The [beta]2-AR expression was investigated using immunohistochemistry and an immunoreactive scoring (IRS) system in 57 different head and neck cancer specimens, and reverse transcriptase-polymerase chain reaction and western blotting in four head and neck cancer cell lines (HNCCLs). Cell viability and proliferation assays were performed using 0, 1, 5 and 10 [micro]M of NE and 1 [micro]M of propranolol in four HNCCLs. The expression of [beta]2-AR was positive in the majority of head and neck cancer tissues (55/57, 96.5%); however, it was signifcantly higher in oral cavity cancer than in pharyngeal cancer (median IRS: 9 vs. 3; P&lt;0.001). All HNCCLs exhibited [beta]2-AR expression, with a higher expression level detected in the oral cavity cancer cell line than in the others. NE stimulated viability (oral cavity, 206%; larynx, 156%; pharynx, 130%; nasal cavity, 137%; 10 [micro]M NE) and proliferation (124, 176, 131 and 127%, respectively) in a dose-dependent manner in all HNCCLs. Conversely, propranolol attenuated such viability (55, 42, 18 and 22%, respectively) and proliferation (22, 40, 61 and 48%, respectively). In conclusion, the viability and proliferation of various head and neck cancers may be directly stimulated by stress and this may be attenuated by [beta]-blockers.</abstract><pub>Spandidos Publications</pub><doi>10.3892/ol.2021.13065</doi></addata></record>
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subjects Analysis
Aprotinin
DNA polymerases
Head and neck cancer
Health aspects
Immunohistochemistry
Propranolol hydrochloride
Scientific equipment and supplies industry
title 2-adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes
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