Determination of Intestinal Enzyme Activities During Infancy Period/Sut Cocuklugu Doneminde Barsak Enzim Aktivitelerinin Belirlenmesi

• Published in: Güncel pediatri 2016-08, Vol.14 (2), p.53
• Main Authors: Orun, Emel, Yalcin, Songul, Lay, Incilay, Dursun, Arzu, Ozkara, Asuman
• Format: Article
• Language: Turkish
• Subjects: Development; Digestive enzymes; Health aspects; Infants; Measurement; Microbiota (Symbiotic organisms); Pediatric research; Physiological aspects
• ISSN: 1304-9054
• DOI: 10.4274/jcp.43434

Introduction: Intestinal enzyme activities are indirect indicators that reflect the existence and metabolic activity of bacteria living in the intestinal flora. The purpose of the study was to measure fecal beta ([beta])-glucuronidase, [beta]-glucosidase and urease enzyme activities and to determine the factors that affect levels in 6 week old and 8 month old babies. Materials and Methods: The study comprised 100 healthy infants at 6 weeks of age. Feces samples were collected from all infants. However, 17 of the feces samples were not included due to the lack of particles in the feces. The same samples were also taken from 35 infants at 8 months of age. Twenty-five of the infants had given feces samples at both 6 weeks and 8 months of age. Urease, [beta]-glucuronidase and [beta]-glucosidase enzyme activities (nmol/[min.sup.-1]/mg-[protein.sup.-1]) were measured. Results: In repeated measures, the levels of [beta]-glucuronidase and urease declined over time and [beta]-glucosidase levels increased. At 8 months of age, higher [beta]-glucuronidase levels were obtained in premature infants. At 6 weeks of age, lower levels of urease were measured in babies who were started breastfeeding at the first hour of life and were bottle-fed. Exclusive breastfeeding had no influence on the intestinal enzyme activities. Conclusions: In early infancy period when microflora is structured, intestinal enzyme activities are important that show indirectly functionality of the microflora. However, it is difficult to highlight what affects the levels of intestinal enzymes because activities vary according to the age. Keywords Intestinal enzyme activity, urease, betaglucuronidase, beta-glucosidase Giris: Barsak enzim aktiviteleri barsak florasinda yasayan bakterilerin varligini ve metabolik aktivitelerini yansitan dolayli belirteclerdir. Calismanin amaci, diskida beta ([beta])-glukuronidaz, [beta]-glukozidaz ve ureaz enzimlerinin aktivite duzeylerini 6 haftalik ve 8 aylik bebeklerde olcmek ve duzeyleri etkileyen faktorleri belirlemektir. Gerec ve Yontem: Calismaya 100 saglikli 6 haftalik bebekler dahil edildi. Tum bebeklerden diski ornegi alindi. Diski orneklerinin 17'si partikulsuz oldugu icin degerlendirme disi birakildi. Calismaya dahil edilen bebeklerin 35'inden 8. ayda ikinci diski ornegi alindi. Bebeklerin 25'inin hem 6 haftalik hem de 8 aylik diski ornekleri vardi. Alinan diski orneklerinde ureaz, [beta]-glukuronidaz ve [beta]-glukozidaz enzim aktiviteleri (nmol/[dk.sup.-1]/