Comparative Study of the [gamma]H2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids

We compared the formation of [gamma]H2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing [sup.3]H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/lite...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2021-12, Vol.172 (2), p.245
Main Authors: Rodneva, S.M, Osipov, A.A, Guryev, D.V, Tsishnatti, A.A, Fedotov, Y.A, Yashkina, E.I, Vorobyova, N.Y, Maksimov, A.A, Kochetkov, O.A, Samoylov, A.S, Osipov, A.N
Format: Article
Language:English
Subjects:
Analysis
Chromatin
Glycine
Nuclear energy
Proline
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Summary:We compared the formation of [gamma]H2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing [sup.3]H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of [gamma]H2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of [sup.3]H-thymidine was more than 2-fold higher than under the influence of [sup.3]H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with [sup.3]H-labeled amino acids showed that [sup.3]H-alanine produced more pronounced effect that [sup.3]H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins. Key Words: [gamma]H2AX; MRC-5; tritium; [sup.3]H-thymidine; [sup.3]H-amino acids
ISSN:0007-4888
DOI:10.1007/s10517-021-05370-6