Isolation and characterization of Henal-anovel Erwinia amylovora bacteriophage

Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriop...

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Bibliographic Details
Published in:FEMS microbiology letters 2020-05, Vol.367 (9), p.1
Main Authors: Besarab, Natalya V, Akhremchuk, Artur E, Zlatohurska, Maryna A, Romaniuk, Liudmyla V, Valentovich, Leonid N, Tovkach, Fedor I, Lagonenko, Alexander L, Evtushenkov, Anatoly N
Format: Article
Language:English
Subjects:
Bacteria, Phytopathogenic
Bacteriophages
Discovery and exploration
Environmental aspects
Genetic aspects
Identification and classification
Physiological aspects
Proteobacteria
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Summary:Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been proposed as environmentally friendly natural antimicrobial agents for fire blight control. Here, we isolated a novel bacteriophage Hena1 with activity against E. amylovora. Further analysis revealed that Hena1 is a narrow-host-range lytic phage belonging to Myoviridae family. Its genome consists of a linear 148,842 bp dsDNA (48.42% GC content) encoding 240 ORFs and 23 tRNA genes. Based on virion structure and genomic composition, Hena1 was classified as a new species of bacteriophage subfamily Vequintavirinae.The comprehensive analysis of Hena1 genome may provide further insights into evolution of bacteriophages infecting plant pathogenic bacteria.
ISSN:0378-1097
1574-6968
DOI:10.1093/femsle/fnaa070