Upregulated expression of transforming growth factor-[beta] receptor I/II in an endemic Osteoarthropathy in China

Background Kashin-Beck disease (KBD) is a chronic, deforming, endemic osteochondropathy that begins in patients as young as 2-3 years of age. The pathogenesis of KBD remains unclear, although selenium (Se) deficiency and T-2 toxin food contamination are both linked to the disease. In the present stu...

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Bibliographic Details
Published in:BMC musculoskeletal disorders 2021-12, Vol.22 (1)
Main Authors: Zhang, Ying, Mu, Yudong, He, Ying, Li, Zhengzheng, Mi, Ge, Liu, Yinan, Zhang, Meng, Wang, Hui, Feng, Yiping, Fang, Qian, Ma, Tianyou, Deng, Xianghua, Chen, Jinghong
Format: Article
Language:English
Subjects:
Development and progression
Diagnosis
Growth factor receptors
Health aspects
Kashin-Beck disease
Risk factors
Transforming growth factors
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Summary:Background Kashin-Beck disease (KBD) is a chronic, deforming, endemic osteochondropathy that begins in patients as young as 2-3 years of age. The pathogenesis of KBD remains unclear, although selenium (Se) deficiency and T-2 toxin food contamination are both linked to the disease. In the present study, we evaluated transforming growth factor-[beta] receptor (TGF-[beta]R I and II) levels in clinical samples of KBD and in pre-clinical disease models. Methods Human specimens were obtained from the hand phalanges of eight donors with KBD and eight control donors. Animal models of the disease were established using Sprague-Dawley rats, which were fed an Se-deficient diet for 4 weeks and later administered the T-2 toxin. Cartilage cellularity and morphology were examined by hematoxylin and eosin staining. Expression and localization of TGF-[beta]RI and II were evaluated using immunohistochemical staining and western blotting. Results In the KBD samples, chondral necrosis was detected based on cartilage cell disappearance and alkalinity loss in the matrix ground substance. In the necrotic areas, TGF-[beta]RI and II staining were strong. Positive percentages of TGF-[beta]RI and II staining were higher in the cartilage samples of KBD donors than in those of control donors. TGF-[beta]RI and II staining was also increased in cartilage samples from rats administered T-2 toxin or fed on Se-deficient plus T-2 toxin diets. Conclusion TGF-[beta]RI and II may be involved in the pathophysiology of KBD. This study provides new insights into the pathways that contribute to KBD development. Keywords: TGF-[beta] receptor II, TGF-[beta] receptor I, Se-deficiency, T-2 toxin, Kashin-Beck disease
ISSN:1471-2474
1471-2474
DOI:10.1186/s12891-021-04939-6