Plasma Cell Proliferation Is Reduced in MyelomaInduced Hypercalcemia and in Co-Culture with Normal Healthy BM-MSCs

Objective: In multiple myeloma (MM), stimulation of osteoclasts and bone marrow (BM) lesions lead to hypercalcemia, renal failure, and anemia. Co-culture of the myeloma cells in both hypocalcemia and hypercalcemia concentrations with bone marrow-mesenchymal stem cells were evaluated. Materials and M...

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Bibliographic Details
Published in:Laboratory medicine 2021-05, Vol.52 (3), p.273
Main Authors: Shiran, Nader Vazifeh, Abroun, Saeid
Format: Article
Language:English
Subjects:
Anemia
B cells
Ethylenediaminetetraacetic acid
Hypercalcemia
Multiple myeloma
Stem cells
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Summary:Objective: In multiple myeloma (MM), stimulation of osteoclasts and bone marrow (BM) lesions lead to hypercalcemia, renal failure, and anemia. Co-culture of the myeloma cells in both hypocalcemia and hypercalcemia concentrations with bone marrow-mesenchymal stem cells were evaluated. Materials and Methods: Viability and survival of myeloma cells were assessed by microculture tetrazolium test and flow cytometric assays. Mesenchymal stem cells (MSCs) were extracted from normal and myeloma patients and were co-cultured with myeloma cells. Results: Myeloma cells showed less survival in both hypocalcaemia and hypercalcemia conditions (P
ISSN:0007-5027
DOI:10.1093/labmed/lmaa060