'Candidatus Chloroploca mongolica' sp. nov. a new mesophilic filamentous anoxygenic phototrophic bacterium

A mesophilic filamentous anoxygenic phototrophic bacterium, designated M50-1, was isolated from a microbial mat of the Chukhyn Nur soda lake (northeastern Mongolia) with salinity of 5-14 g/L and pH 8.0-9.3. The organism is a strictly anaerobic phototrophic bacterium, which required sulfide for photo...

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Bibliographic Details
Published in:FEMS microbiology letters 2021-08, Vol.368 (16), p.1
Main Authors: Bryantseva, Irina A, Grouzdev, Denis S, Krutkina, Maria S, Ashikhmin, Aleksandr A, Kostrikina, Nadezda A, Koziaeva, Veronika V, Gorlenko, Vladimir M
Format: Article
Language:English
Subjects:
Analysis
Bacteria, Photosynthetic
Genetic aspects
Genomes
Genomics
Identification and classification
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Summary:A mesophilic filamentous anoxygenic phototrophic bacterium, designated M50-1, was isolated from a microbial mat of the Chukhyn Nur soda lake (northeastern Mongolia) with salinity of 5-14 g/L and pH 8.0-9.3. The organism is a strictly anaerobic phototrophic bacterium, which required sulfide for phototrophic growth. The cells formed short undulate trichomes surrounded by a thin sheath and containing gas vesicles. Motility of the trichomes was not observed. The cells contained chlorosomes. The antenna pigments were bacteriochlorophyll d and p- and y -carotenes. Analysis of the genome assembled from the metagenome of the enrichment culture revealed all the enzymes of the 3-hydroxypropionate bi-cycle for autotrophic [CO.sub.2] assimilation. The genome also contained the genes encoding a type IV sulfide:quinone oxidoreductase (sqrX). The organism had no ni/HDBK genes, encoding the proteins of the nitrogenase complex responsible for dinitrogen fixation. The DNA G + C content was 58.6%. The values for in silico DNA-DNA hybridization and average nucleotide identity between M50-1 and a closely related bacterium 'Ca. Chloroploca asiatica' B7-9 containing bacteriochlorophyll c were 53.4% and 94.0%, respectively, which corresponds to interspecies differences. Classification of the filamentous anoxygenic phototrophic bacterium M50-1 as a new 'Ca. Chloroploca' species was proposed, with the species name 'Candidatus Chloroploca mongolica' sp. nov.
ISSN:0378-1097
1574-6968
DOI:10.1093/femsle/fnabl07