Molecular identification of genus Pipistrellus da regiao de Fata, Paquistao

• Published in: Brazilian journal of biology 2023-01, Vol.83
• Main Authors: Idnan, M, Javid, A, Tayyab, M, Hussain, A, Mansoor, S, Bukhari, S.M, Irfan, Shahbaz, M, Rehman, K. ur, Andleeb, S, Azam, S.M, Ali, W
• Format: Article
• Language: English
• ISSN: 1519-6984
• DOI: 10.1590/1519-6984.246322

A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhlii lepidus (n=10) was 43[+ or -]0.11 mm and 45[+ or -]1.1 respectively. Morphologically identified Pipistrellus kuhlii confirmed as Pipistrellus kuhlii lepidus based on 16S rRNA sequences. The DNA sequences were submitted to GenBank and accession numbers were obtained (MN 719478 and MT430902). The available 16S rRNA gene sequences of Pipistrellus coromondra and Pipistrellus kuhlii lepidus were retrieved from NCB1 and incorporated in N-J tree analysis. Overall, the interspecific genetic variations among Pipistrellus coromondra and Pipistrellus kuhlii lepidus were 8% and 1% respectively. 1n our recommendation, a comprehensive molecular identification of bats is need of hour to report more cryptic and new species from Pakistan. Keywords: Pipistrellus, Pipistrellus kuhlii lepidus, Pipistrellus kuhlii, Pipistrellus coromondra. Um total de 10 especimes foi capturado em locais selecionados da Bajaur Agency FATA, Paquistao, usando redes de neblina. Os especimes capturados foram identificados morfologicamente e varias medidas morfometricas foram realizadas. O comprimento da cabeca e do corpo (HB) de Pipistrellus coromondra e Pipistrellus kuhlii lepidus (n = 10) foi de 43 [+ or -] 0,11 mm e 45 [+ or -] 1,1, respectivamente. Pipistrellus kuhlii identificado morfologicamente e confirmado como Pipistrellus kuhlii lepidus com base em sequencias de rRNA 16S. As sequencias de DNA foram submetidas ao GenBank e os numeros de acesso foram obtidos (MN 719478 e MT430902). As sequencias do gene 16S rRNA disponiveis de Pipistrellus coromondra e Pipistrellus kuhlii lepidus foram recuperadas do NCB1 e incorporadas na analise da arvore N-J. No geral, as variacoes geneticas interespecificas entre Pipistrellus coromondra e Pipistrellus kuhlii lepidus foram de 8% e 1%, respectivamente. Em nossa recomendacao, uma identificacao molecular abrangente de morcegos precisa de uma hora para relatar mais especies cripticas e novas do Paquistao. Palavras-chave: Pipistrellus, Pipistrellus kuhlii lepidus, Pipistrellus kuhlii, Pipistrellus coromondra.