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Therapeutic potential of stem cell and melatonin on the reduction of C[Cl.sub.4]-induced liver fibrosis in experimental mice model/ Potencial terapeutico de celulas-tronco e melatonina na reducao da fibrose hepatica induzida por C[Cl.sub.4] no modelo de ratos experimentais

Liver fibrosis is initial stage of any chronic liver disease and its end stage is develops into cirrhosis. Chronic liver diseases are a crucial global health issue and the cause of approximately 2 million deaths per year worldwide. Cirrhosis is currently the 11th most common cause of death globally....

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Bibliographic Details
Published in:Brazilian journal of biology 2024-01, Vol.84
Main Authors: Rafiq, H, Ayaz, M, Khan, H.A, Iqbal, M, Quraish, S, Afridi, S.G, Khan, A, Khan, B, Sher, A, Siraj, F, Shams, S
Format: Article
Language:English
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Summary:Liver fibrosis is initial stage of any chronic liver disease and its end stage is develops into cirrhosis. Chronic liver diseases are a crucial global health issue and the cause of approximately 2 million deaths per year worldwide. Cirrhosis is currently the 11th most common cause of death globally. Mesenchymal stem cell (MSCs) treatment is the best way to treat acute and chronic liver disease. The aim of this study is to improve the therapeutic potential of MSCs combined with melatonin (MLT) to overcome C[Cl.sub.4]-induced liver fibrosis and also investigate the individual impact of melatonin and MSCs against C[Cl.sub.4]-induced liver impairment in animal model. Female BALB/c mice were used as C[Cl.sub.4]-induced liver fibrotic animal model. Five groups of animal model were made; negative control, Positive control, C[Cl.sub.4]+MSCs treated group, C[Cl.sub.4]+MLT treated group and C[Cl.sub.4]+MSCs+MLT treated group. Cultured MSCs from mice bone marrow were transplanted to C[Cl.sub.4]-induced liver injured mice model, individually as well as together with melatonin. Two weeks after MSCs and MLT administration, all groups of mice were sacrificed for examination. Morphological and Histopathological results showed that combined therapy of MSCs+MLT showed substantial beneficial impact on C[Cl.sub.4]-induced liver injured model, compared with MSCs and MLT individually. Biochemically, considerable reduction was observed in serum bilirubin and ALT levels of MLT+MSC treated mice, compared to other groups. PCR results shown down-regulation of Bax and up-regulation of Bcl-xl and Albumin, confirm a significant therapeutic effect of MSCs+MLT on C[Cl.sub.4]-induced liver fibrosis. From the results, it is concluded that combined therapy of MSCs and MLT show strong therapeutic effect on C[Cl.sub.4]-induced liver fibrosis, compared with MSCs and MLT individually.
ISSN:1519-6984
1678-4375
DOI:10.1590/1519-6984.253061