Increased Yield of Residual [gamma]H2AX Foci in p53-Deficient Human Lung Carcinoma Cells Exposed to Subpicosecond Beams of Accelerated Electrons

• Published in: Bulletin of experimental biology and medicine 2022-04, Vol.172 (6), p.756
• Main Authors: Vorobyeva, N. Yu, Babayan, N.S, Grigoryan, B.A, Sargsyan, A.A, Khondkaryan, L.G, Apresyan, L.S, Chigasova, A.K, Yashkina, E.I, Guryev, D.V, Rodneva, S.M, Tsishnatti, A.A, Fedotov, Yu. A, Arutyunyan, R.M, Osipov, A.N
• Format: Article
• Language: English
• Subjects: Cancer; Carcinoma; Electrons; Lung cancer; Tumor proteins
• ISSN: 0007-4888
• DOI: 10.1007/s10517-022-05472-9

We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone ([gamma]H2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate during the pulse 16 GGy/s) and quasi-continuous (energy 3.6 MeV) beams of accelerated electrons in a dose range of 0.5-10.0 Gy. The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual [gamma]H2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons. Key Words: subpicosecond electron irradiation; DNA double strand breaks; residual [gamma]H2AX foci; p53; human lung carcinoma