Anti-cell growth and anti-cancer stem cell activity of the CDK4/6 inhibitor palbociclib in breast cancer cells

• Published in: Breast cancer (Tokyo, Japan) Japan), 2020-05, Vol.27 (3), p.415-425
• Main Authors: Kishino, Emi, Ogata, Ryohei, Saitoh, Wataru, Koike, Yoshikazu, Ohta, Yusuke, Kanomata, Naoki, Kurebayashi, Junichi
• Format: Article
• Language: English
• Subjects: Antimitotic agents; Antineoplastic agents; Apoptosis; Biomarkers, Tumor - metabolism; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Cancer cells; Cancer Research; Care and treatment; Cell Movement; Cell Proliferation; Cyclin-Dependent Kinase 4 - antagonists & inhibitors; Cyclin-Dependent Kinase 6 - antagonists & inhibitors; Epidermal growth factor; Estradiol; Female; Gene Expression Regulation, Neoplastic - drug effects; Growth; Humans; Medicine; Medicine & Public Health; Neoplastic Stem Cells - drug effects; Neoplastic Stem Cells - metabolism; Neoplastic Stem Cells - pathology; Oncology; Original Article; Piperazines - pharmacology; Protein Kinase Inhibitors - pharmacology; Pyridines - pharmacology; Stem cells; Surgery; Surgical Oncology; Tumor Cells, Cultured
• ISSN: 1340-6868; 1880-4233
• DOI: 10.1007/s12282-019-01035-5

Background A cyclin-dependent kinase (CDK) 4/6 inhibitor, palbociclib, has been used to treat patients with estrogen receptor (ER)-positive (+) and human epidermal growth factor receptor (HER) 2-negative (−) advanced breast cancer. To investigate the mechanisms underlying the antitumor activity of palbociclib, we conducted a preclinical study on the anti-cell growth and anti-cancer stem cell (CSC) activity of palbociclib in breast cancer cells. Methods The effects of palbociclib on Rb phosphorylation, cell growth, cell cycle progression, apoptosis, cell senescence and the proportion of CSCs were investigated in five human breast cancer cell lines of different subtypes. To investigate the mechanisms of the anti-CSC activity of palbociclib, small-interfering RNAs for CDK4 and/or CDK6 were used. Palbociclib dose-dependently reduced Rb phosphorylation and cell growth in association with G1-S cell cycle blockade and the induction of cell senescence, but without increased apoptosis, in all breast cancer cell lines. Results The anti-cell growth activity of palbociclib widely differed among the cell lines. Palbociclib also dose-dependently reduced the CSC proportion measured by three different assays in four of five cell lines. The inhibition of CDK4 expression, but not CDK6 expression, reduced the increased proportion of putative CSCs induced by estradiol in ER (+)/HER2 (−) cell lines. Conclusions These results suggest that palbociclib exhibits significant anti-cell growth and anti-CSC activity in not only ER (+) breast cancer cell lines but also ER (−) cell lines. CDK4 inhibition induced by palbociclib may be responsible for its anti-CSC activity.