Electrochemiluminescence paper-based screen-printed electrode for HbA1c detection using two-dimensional zirconium metal-organic framework/Fe.sub.3O.sub.4 nanosheet composites decorated with Au nanoclusters

Glycated hemoglobin (HbA1c) is one of the most popular biomarkers which can be utilized for the diagnosis and control of diabetes in clinical practice. In this study, a sandwich paper-based electrochemiluminescence (ECL) biosensor has been developed using the zirconium metal-organic framework/Fe.sub...

Full description

Saved in:
Bibliographic Details
Published in:Mikrochimica acta (1966) 2021-09, Vol.188 (9)
Main Authors: Ahmadi, Anita, Khoshfetrat, Seyyed Mehdi, Kabiri, Shima, Dorraji, Parisa Seyed, Larijani, Bagher, Omidfar, Kobra
Format: Article
Language:English
Subjects:
Analysis
Detectors
Diabetes therapy
Ethylenediaminetetraacetic acid
Glycosylated hemoglobin
Monoclonal antibodies
Zirconium
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glycated hemoglobin (HbA1c) is one of the most popular biomarkers which can be utilized for the diagnosis and control of diabetes in clinical practice. In this study, a sandwich paper-based electrochemiluminescence (ECL) biosensor has been developed using the zirconium metal-organic framework/Fe.sub.3O.sub.4(trimethyl chitosan)/gold nanocluster (Zr-MOF/Fe.sub.3O.sub.4(TMC)/AuNCs) nanocomposite as tracing tag to label anti-HbA1c monoclonal antibody and reduced graphene oxide (rGO) as immobilization platform of sensing element. The screen-printed electrodes (SPEs) were constructed and modified by sputtering a thick layer of gold on the paper substrate, followed by electrochemical reduction of aminophenylboronic acid (APBA)-functionalized GO to rGO/APBA, respectively. Different types of surface analysis methods were applied to characterize the Zr-MOF/Fe.sub.3O.sub.4(TMC)/AuNCs nanomaterials fabricated. Finally, antibody-labeled Zr-MOF/Fe.sub.3O.sub.4(TMC)/AuNCs nanocomposites were subjected to HbA1c in the sample and on the paper-based SPE. Quantitative measurement of HbA1c was performed using ECL and cyclic voltammetry (CV) over a potential range of - 0.2 to 1.7 V vs gold reference electrode with a sweep rate of 0.2 V.s.sup.-1 in the presence of triethylamine as a co-reactant after sandwiching the HbA1c target between antibody and APBA on the sensing area. This immunosensor demonstrated the desirable assay performance for HbA1c with a wide response range from 2 to 18% and a low detection limit (0.072%). This new strategy provides an effective method for high-performance bioanalysis and opens avenues for the development of high-sensitive and user-friendly device.
ISSN:0026-3672
DOI:10.1007/s00604-021-04959-y