CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression

Objectives: This study aims to investigate the role of cluster of differentiation 14 (CD14) expressed monocytes and soluble CD14-mediated pathway in the synovial inflammation of knee osteoarthritis (OA). Patients and methods: Between May 2012 and July 2013, a total of 35 patients with knee OA (9 mal...

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Published in:Archives of rheumatology 2022-09, Vol.37 (3), p.335-343
Main Authors: Lee, Ha-Reum, Lee, Sunyoung, Yoo, In Seol, Yoo, Su-Jin, Kwon, Mi-Hye, Joung, Chung-il, Park, Ji Ah, Kang, Seong Wook, Kim, Jinhyun
Format: Article
Language:English
Subjects:
Analysis
B cells
Development and progression
Immunohistochemistry
Inflammation
Interleukins
Medical equipment and supplies industry
Medical test kit industry
Osteoarthritis
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Summary:Objectives: This study aims to investigate the role of cluster of differentiation 14 (CD14) expressed monocytes and soluble CD14-mediated pathway in the synovial inflammation of knee osteoarthritis (OA). Patients and methods: Between May 2012 and July 2013, a total of 35 patients with knee OA (9 males, 26 females; mean age: 66.3 [+ or -] 8.8 years; range, 52 to 79 years) were included in this cross-sectional study. Synovial fluid was obtained from knee joints of 35 OA patients. The [CD14.sup.+] monocytes from synovial fluid mononuclear cells (SFMCs) were isolated using the MACS. The fibroblast-like synoviocytes (FLSs) isolated from knee joint tissue were incubated with recombinant CD14 and lipopolysaccharide (LPS) for 24 h. Cytokine profiling was performed with the Luminex[R] Performance Assay or magnetic bead panel kit. The expression of CD14 and CD16 was analyzed by immunohistochemistry and flow cytometry. Results: The concentration of sCD14 in synovial fluid was correlated with the interleukin-6 (IL-6) level (n=35) (p=0.654, p
ISSN:2148-5046
2618-6500
DOI:10.46497/ArchRheumatol.2022.9078