Genomic Characterization of International High-Risk Clone ST410 IEscherichia coli/I Co-Harboring ESBL-Encoding Genes and Ibla/I[sub.NDM-5] on IncFIA/IncFIB/IncFII/IncQ1 Multireplicon Plasmid and Carrying a Chromosome-Borne Ibla/I[sub.CMY-2] from Egypt

• Published in: Antibiotics (Basel) 2022-07, Vol.11 (8)
• Main Authors: Mohamed, Nelly M, Zakaria, Azza S, Edward, Eva A
• Format: Article
• Language: English
• Subjects: Beta lactamases; Biotechnology industry; Cloning; Genes; Imipenem
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics11081031

The accelerated dispersion of multidrug-resistant (MDR) Escherichia coli due to the production of extended-spectrum β-lactamases (ESBLs) or AmpC enzymes has been noted in Egypt, presenting a serious treatment challenge. In this study, we investigate the prevalence of ESBLs and AmpC enzymes among 48 E. coli isolates collected from patients with urinary tract infections admitted to a teaching hospital in Alexandria. Phenotypic and genotypic methods of detection are conducted. Isolates producing both enzymes are tested for the mobilization of their genes by a broth mating experiment. Whole genome sequencing (WGS) is performed for isolate EC13655. The results indicate that 80% of the isolates are MDR, among which 52% and 13% were ESBL and AmpC producers, respectively. Conjugation experiments fail to show the mobilization of bla[sub.CMY-2] in EC13655, which was chosen for WGS. In silico analysis reveals that the isolate belongs to a ST410-H24Rx high-risk clone. It coharbors the ESBL-encoding genes bla[sub.CTX-M-15], bla[sub.TEM-1], bla[sub.OXA-1] and bla[sub.NDM-5] on an IncFIA/IncFIB/IncFII/IncQ1 multireplicon plasmid. The chromosomal location of bla[sub.CMY-2] is detected with a flanking upstream copy of ISEcp1. This chromosomal integration of bla[sub.CMY-2] establishes the stable maintenance of the gene and thus, necessitates an imperative local surveillance to reduce further spread of such strains in different clinical settings.