Decreased Presence of Mast Cells in the Bursa Premacularis of Proliferative Diabetic Retinopathy

Introduction: We previously reported that the intravitreal activities of chymase and tryptase were more increased in the patients with macular hole (MH) and epiretinal membrane (ERM) than in those with proliferative diabetic retinopathy (PDR) and that the source of these serine proteases might be ma...

Full description

Saved in:
Bibliographic Details
Published in:Ophthalmic research 2021, Vol.64 (6), p.1002-1012
Main Authors: Ikeda, Tsunehiko, Nakamura, Kimitoshi, Morishita, Seita, Sato, Takaki, Horie, Taeko, Kida, Teruyo, Oku, Hidehiro, Takai, Shinji, Jin, Denan
Format: Article
Language:English
Subjects:
Antibodies
Business performance management
Cells
Chymases
Diabetes Mellitus
Diabetic Retinopathy
Epiretinal Membrane
Hematoxylin
Humans
Mast Cells
Proteases
Research Article
Retinal Perforations
Tolonium Chloride
Tryptases
Viral antibodies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Introduction: We previously reported that the intravitreal activities of chymase and tryptase were more increased in the patients with macular hole (MH) and epiretinal membrane (ERM) than in those with proliferative diabetic retinopathy (PDR) and that the source of these serine proteases might be mast cells in the bursa premacularis (BPM). The purpose of this study was to compare the density of mast cells in BPM samples obtained from MH, ERM, and PDR patients. Methods: BPM and vitreous core samples were first collected during vitrectomy from eyes afflicted with vitreoretinal diseases (MH: 6 eyes, ERM: 3 eyes, and PDR: 9 eyes), and then were stained with hematoxylin, toluidine blue, antibodies against chymase and tryptase, and a terminal deoxynucleotidyl transferase dUTP nick end labeling assay kit. Results: Hematoxylin nuclear staining showed fewer positive-staining cells in the BPM samples obtained from PDR patients than in those obtained from MH and ERM patients. Toluidine blue staining of the BPM revealed metachromasia in the mast cells of the patients with MH and ERM, but not those of the patients with PDR. In addition, immunostaining using anti-chymase and anti-tryptase antibodies showed that the BPM samples were more intensely stained than the vitreous core samples from the patients with MH and ERM and that both tissue samples were poorly stained in the patients with PDR. The apoptotic cells were more frequently observed in the BPM samples from patients with MH than in those from patients with PDR. Conclusions: These findings indicated that lower activities of chymase and tryptase in the vitreous of PDR patients appeared to be attributable to the decreased presence of mast cells in the BPM. The lack of mast cells in the BPM might be related to the pathogenesis of PDR.
ISSN:0030-3747
1423-0259
DOI:10.1159/000518438