Expression of MicroRNAs miR-126, miR-133b and miR-221 Associated with Apoptosis in Rats Submitted to Focal Cerebral Ischemia and Physical Exercise/Expresión de MicroRNAs miR-126, miR-133b y miR-221 Asociados a Apoptosis en Ratas Sometidas a Isquemia Cerebral Focal y Ejercicio Físico

• Published in: International journal of morphology 2022-11, Vol.40 (6), p.1518
• Main Authors: Figueiredo, Rafael Zimak, Cirino, Mucio Luiz de Assis, Porsani, Lucas Barbosa, Neto, Fermino Sanches Lizarte, Novais, Paulo Cezar, Nunes, Maria Julia, Lellis, Janaína Regina, da Silva, Jairo Pinheiro, Tazima, Maria de Fátima Galli Sorita, Durand, Marina de Toledo, Fazan, Valeria Paula Sassoli, Colli, Benedicto Oscar, da Cunha Tirapelli, Daniela Pretti, Tirapelli, Luis Fernando
• Format: Article
• Language: English
• ISSN: 0717-9367

Stroke is one of the main causes of death and disability worldwide. The great impact on the quality of life of the population and on the health system justifies that we seek relevant alternatives to reduce the incidence and improve the treatment and recovery of patients affected by this disease. Physical exercise appears as an important tool in this scenario, being already pointed out as a possible therapeutic approach for the prevention of non-contagious chronic diseases. In this context, biomarkers such as miRNAs that respond to physical exercise and are directly related to several epigenetic mechanisms appear. Therefore, explaining the molecular mechanisms involved during physical exercise will lead to a better understanding of each stimulus and the dose to be used to better respond to each situation, thus being a promising approach for the evolution of prescription and control of training and processes recovery from various diseases, including stroke. Forty-eight Wistar rats were used, divided into four experimental groups: control group, ischemia group, physical exercise group and exercise + ischemia group. Real-time PCR methodology was used to analyze the expression of miRNAs: miR-126, miR-133b and miR-221. In our study we observed a significant difference in the expression of miR-221 between the control group and the others groups. However, microRNAs: miR-126 and miR-133b do not show significant differences in expression between groups. KEY WORDS: Cerebral ischemia; Physical exercise; MicroRNAs; Apoptosis. El ictus es una de las principales causas de muerte y discapacidad en todo el mundo. El gran impacto en la calidad de vida de la población y en el sistema de salud justifica buscar alternativas pertinentes para reducir la incidencia y mejorar el tratamiento y recuperación de los pacientes afectados por esta enfermedad. El ejercicio físico aparece como una herramienta importante en este escenario, siendo ya señalado como un posible abordaje terapéutico para la prevención de enfermedades crónicas no contagiosas. En este contexto, aparecen biomarcadores como los miRNAs que responden al ejercicio físico y están directamente relacionados con varios mecanismos epigenéticos. Por lo tanto, explicar los mecanismos moleculares involucrados durante el ejercicio físico conducirá a una mejor comprensión de cada estímulo y la dosis a utilizar para responder mejor a cada situación, siendo así un enfoque prometedor para la evolución de la prescripción, el control