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DISCRETE BANDS SEEN AT THE POINT OF APPLICATION IN ALL LANES OF IMMU NO FIXATION ELECTROPHORESIS

Objective: To analyze a rare case of discrete bands seen at the point of application in all lanes of Immunofixation Electrophoresis. Material and Method: A serum sample from 61 years male patient was requested for serum Protein Electrophoresis (SPE) and Serum Immunofixation Electrophoresis (IFE). Th...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.27 (S1), p.S73
Main Authors: Gupta, Rajeev, Kumar, Dilip, Das, Poonam, Dayal, Nitin, Arya, Anupama, Abrari, Andleeb, Garg, Twinkle
Format: Article
Language:English
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Summary:Objective: To analyze a rare case of discrete bands seen at the point of application in all lanes of Immunofixation Electrophoresis. Material and Method: A serum sample from 61 years male patient was requested for serum Protein Electrophoresis (SPE) and Serum Immunofixation Electrophoresis (IFE). The SPE and Serum IFE were performed on agarose gel kit provided by Helena Biosciences. The Electrophoretogram was scanned by scanner and the densities of the discrete bands were measured at 620 nm by using Platinum software, Helena Biosciences. Serum Protein was analyzed by DXC 800 Auto analyzer, Beckman Coulter. In addition Immunoglobulin Profile (IgG, IgA and IgM), Beta 2 Micro globulin, 24 hours Urine IFE, Bence Jones Protein, Complete Blood Count (CBC), Bone marrow Aspirate and Biopsy, Flow Cytometry for CLL and Left Axillary lymph node biopsy with IHC were also done. Results: Biclonal 'M' spike was seen in Gamma region of SPE lane. In all other lanes (i.e. IgG, IgM, IgA, K, A) a discrete Band was seen at the Point of application. Biclonal 'M' Band seen in Gamma region was of IgM kappa type. Total protein was 10.4 g/dL. IgM was very high 5525 mg/ dl {60-280}. Serum Beta 2 Micro globulin was 8.44 mg/L {0.9-3.0}. Rheumatoid Factor (RF) was negative. Bence Jones Protein was negative. Histo Biopsy and Immunohistochemistry (IHC) from Left Axillary lymph node was reported as Lympho-plasmacytic Lymphoma (Low grade B- cell Non Hodgkin Lymphoma). Conclusion: Final Diagnosis of Waldenstrom's macroglobulinemia was made. The discrete Band seen in all lanes of Serum IFE was due to non specific staining of precipitated IgM proteins which got adhered to gel matrix of Agarose and was stained by Acid Blue Stain.
ISSN:0970-1915