Detection of Urinary Vitamin Levels in Chronic Moderate Alcohol Consumers by HPLC-Q-TOF-MS and Their Reversal Effects by Tinosporacordifolia

Heavy alcohol intake depletes the plasma vitamins due to hepatotoxicity and decreased intestinal absorption. However, moderate alcohol intake is thought to be healthy. Therefore, effects of moderate alcohol intake on liver and intestinal absorption were studied using urinary vitamin levels as marker...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.30 (S1), p.S75
Main Authors: Sharma, Bhawana, Dabur, Rajesh
Format: Article
Language:English
Subjects:
Analysis
Drinking of alcoholic beverages
High performance liquid chromatography
Liver diseases
Vitamins
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Summary:Heavy alcohol intake depletes the plasma vitamins due to hepatotoxicity and decreased intestinal absorption. However, moderate alcohol intake is thought to be healthy. Therefore, effects of moderate alcohol intake on liver and intestinal absorption were studied using urinary vitamin levels as markers. Furthermore, effects of Tinosporacordifolia water extract (TCE) on vitamin excretion and intestinal absorption were also studied. In the study, asymptomatic moderate alcoholics (n = 12) without chronic liver disease and healthy volunteers (n = 14) of mean age 39 [+ or -] 2.2 (mean [+ or -] SD) were selected and divided into three groups. TCE treatment was performed for 14 days. The blood and urine samples were collected on day 0 and 14 after treatment with TCE and analyzed. Alcoholics samples showed significant increased levels of [gamma]GT, AST, ALT, TGL, CHL, HDL and LDL (p < 0.05) but their level get down-regulated after TCE intervention. Multivariate analysis of metabolites without missing values showed an increased excretion of 7-dehydrocholesterol, orotic acid, pyridoxine, lipoamide and niacin and TCE intervention depleted their levels (p < 0.05). In contrast, excretion of biotin, xanthine, vitamin D2 and 2-O-p-coumaroyltartronic acid (CA, an internal marker of intestinal absorption) were observed to be decreased in alcoholic samples; however TCE intervention restored the CA and biotin levels. Vitamin metabolism biomarkers i.e. homocysteine, xanthurenic acid, etc. were also normalized after TCE intervention. Therefore, overall data depict that moderate alcohol intake is also hepatotoxic and decrease intestinal absorption. However TCE treatment effectively increased the intestinal absorption and retaining power of liver that regulated alcohol induced multivitamin deficiency.
ISSN:0970-1915