The influence of Nanocrystalline Cerium Dioxide on Motor Function of Stomach and Colon in Rats

• Published in: Digestive diseases and sciences 2022-05, Vol.59 (8), p.1656
• Main Authors: Iefimenko, O, Savchenko, Iu, Falalyeyeva, T, Beregova, T, Spivak, M
• Format: Article
• Language: English
• Subjects: Acetylcholine; Cerium; Gastrointestinal agents
• ISSN: 0163-2116
• DOI: 10.1007/s10620-014-3278-0

Introduction: Constipations arise more often in old than in young people. Modern laxatives do not solve this problem. Most of them have side effects and some of them cause addiction. Therefore, the research of new laxatives is still an actual problem of modern medicine. The aim of our study was to investigate the influence of nanocrystalline cerium dioxide (NCD) on spontaneous motor activity (SMA) of stomach and colon in vivo and contractive activity of smooth muscles in vitro in rats. Methods: The investigations were carried out on two groups of rats of 3 and 24 month old. All rats were divided in several groups: I--control group, II--rats were intragastric administered 3 ml of water once a day, III--rats were intragastric administered 3 ml/kg stabilizing solution and IV group of rats, were intragastric administered NCD in dose 1 mmol/ml once a day during 10 days. In a day of last injection of water or NCD SMA was recorded by ballonographic method. The contractive activity of smooth muscles was studied by strain method and with usage of classic stimulants: hyperpotassium isotonic solution (HPS) and acetylcholine (Ach). Motor index (MI) of SMA was determined for a minute period and for contractive activity of smooth muscles: amplitude, phase contraction (Vnc) and relaxation (Vnr) of maximum normalized velocity. Results: With ageing the MI decreased in stomach and colon of rats. NCD increased MI in both group, but in old group this effect was higher. In stomach all parameters of HPS- and Ach-stimulated contractions did not change with age. NCD increased Vnc by 138% (p < 0.01), Vnr by 128% (p < 0.01) under HPS-stimulation and Vnr by 90% (p < 0.001) under Ach-stimulation of stomach. In colon Vnc and Vnr decreased with age by 47.8% (p < 0.01) and 81% (p < 0.01) under HPS-stimulation respectively. These parameters were increased by 166.8% and 644% (p < 0.001) in old rats after NCD administration, accordingly. NCD prolonged Vnc by 17% in young rats and 34.3% (p < 0.05) under Ach-stimulation in old rats. Conclusion: NCD showed prokinetic effect. These results can be background for creation of new laxatives on the basis of NCD.