Isolation and characterization of a compound from the leaves of Ageratum conyzoides linn responsible for anti-gastric ulcer activity in albino rats

Introduction: Ageratum conyzoides Linn (A. conyzoides L.) is a medicinal plant under the family asteraceae. Modern researchers claimed that A. conyzoides L. has antibacterial and anti-tumor activity. It also has wound healing effect. We have noted anti gastric ulcer activity of the leaves of A. cony...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2016-12, Vol.31 (S1), p.S42
Main Authors: Mitra, Prasenjit, Sharma, Praveen, Ghosh, Tanaya, Mitra, Prasanta Kumar
Format: Article
Language:English
Subjects:
Animal experimentation
Hydrolysis
Indomethacin
Mass spectrometry
Medicinal plants
Medicine, Botanic
Medicine, Herbal
Nuclear magnetic resonance spectroscopy
Ranitidine
Stomach ulcer
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Summary:Introduction: Ageratum conyzoides Linn (A. conyzoides L.) is a medicinal plant under the family asteraceae. Modern researchers claimed that A. conyzoides L. has antibacterial and anti-tumor activity. It also has wound healing effect. We have noted anti gastric ulcer activity of the leaves of A. conyzoides L. in indomethacin induced gastric ulcer in albino rats. Aims & Objectives: To isolate and characterize the active ingredient from the leaves of A. conyzoides L. responsible for anti-gastric ulcer activity in albino rats Materials & Methods: By solvent extractions, acid hydrolysis, chromatography followed by crystallization, a compound was isolated from the leaves of A. conyzoides L. To note anti gastric ulcer activity of the isolated compound, if any, Sprague Dawley male rats were divided into four groups: Control, Indomethacin (10 mg/kg) treated, Indomethacin (10 mg/kg) along with isolated compound (50 mg/kg), Indomethacin (10 mg/kg) along with Ranitidine (50 mg/kg). After the completion of experiment, rats were sacrificed. Stomachs were taken out and examined for ulcers for evaluation of ulcer index. Characterization of the compound was done by infrared spectroscopy, mass spectroscopy and nuclear magnetic resonance studies. Results: Compound isolated from Ageratum conyzoides L significantly reduced ulcer index in rats induced by indomethacin. Results were comparable to that of ranitidine, a standard anti-ulcer drug. Analysis of spectral data revealed that the isolated compound was 1,3,5-trihydroxy-7-methylanthracene-9,10-dione.s Conclusion: 1,3,5-trihydroxy-7-methylanthracene-9,10-dione present in the leaves of A. conyzoides L. was responsible for anti-gastric ulcer activity in indomethacin induced gastric ulcer in albino rats.
ISSN:0970-1915