Dietary folate and vitamin B12 levels during pregnancy induce change in expression of imprinting genes in maternal and fetal tissues

Background: The altered ratio of folic acid and vitamin B12 in maternal diet during pregnancy influences the DNA methylation patterns of offspring. Objective: The present study was planned to study the effect of dietary folate and B12 during pregnancy on expression of imprinting genes in maternal an...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2016-12, Vol.31 (S1), p.S116
Main Authors: Mahajan, Aatish, Sapehia, Divika, Mohan, Raj P.S, Kaur, Jyotdeep
Format: Article
Language:English
Subjects:
Analysis
Book publishing
Fetus
Folic acid
Genes
Genetic research
Growth
Methylation
Mice
Planning
Pregnancy
Pregnant women
RNA
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Summary:Background: The altered ratio of folic acid and vitamin B12 in maternal diet during pregnancy influences the DNA methylation patterns of offspring. Objective: The present study was planned to study the effect of dietary folate and B12 during pregnancy on expression of imprinting genes in maternal and fetal tissues. Material and Methods: Four week old C57BL/6 female mice for F0 generation were divided into nine groups NBNF, NBFO, NBFD, BDNF, BDFD, BDFO, BONF, BOFO and BOFD and were fed diet for six to eight weeks while male mice were fed normal diet. On day 20 of gestation mice were sacrificed, fetal and maternal tissues were isolated whereas some of the pregnant mice were kept for F1 generation. Both males and females fetuses in F1 generation were given same diet as fed to their mothers for 6 weeks. Mating was performed within same groups and fetuses born were denoted as F2 generation. The mRNA expression of some imprinting genes Igf2, KCNQIOT1, H19, Igf2R and folate transporters RFC, PCFT, FRa were analyzed by Real-Time PCR. Results: In B12 deficiency, growth retardation was observed in fetuses born in F2 generation. In maternal and fetal tissues H19, which is a negative regulator of growth, was significantly up regulated and IGF2, a growth promoting gene showed a 70 fold decrease in placenta under folate & B12 deficient conditions. IGF2R in folate deficiency and over-supplementation of B12 was down regulated. KCNQ1OT1 showed loss of expression in male fetal tissues in folate & B12 deficiency whereas its expression was increased in female fetal tissue. There was a significant increase in expression of PCFT and FR? in folate deficient groups. Conclusion: There is a definite role of maternal folate and B12 deficiency towards fetal growth and thus may be involved in establishment of proper imprints. Abbreviations: NB: Normal B12, NF: Normal folate, FD: Folate deficient, FO: Folate over supplementation, BD: B12 deficient, BO: B12 over supplementation.
ISSN:0970-1915