Bacopa Monnieri Mode of Actionagainst MPTP Induced Parkinson's Disease Mice Model: An In Silico And In- Vivo Study

INTRODUCTION: Parkinson's disease (PD) is a progressive neurodegenerative movement disorder, however its management is still challenging. Currently studies are being conducted for efficient cure in PD, by using herbal plant. In the present study, an effort has been made to explore neuroprotecti...

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Published in:Indian journal of clinical biochemistry 2022-05, Vol.32 (S1), p.S102
Main Authors: Singh, Babita, Pandey, Shivani, Yadav, Satyndra Kumar, Ubaid, Saba, Mahdi, Abbas Ali
Format: Article
Language:English
Subjects:
Acetic acid
Analysis
Antioxidants
Enzymes
Medical research
Medicine, Experimental
Mice
Organic acids
Protein binding
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Summary:INTRODUCTION: Parkinson's disease (PD) is a progressive neurodegenerative movement disorder, however its management is still challenging. Currently studies are being conducted for efficient cure in PD, by using herbal plant. In the present study, an effort has been made to explore neuroprotective mode of action of herbal plant Bacopa monnieri in MPTP induced neurodegeneration against mice model of PD through in silico and in vivo study. MATERIAL AND METHODS: Experimental mice were given 40mg/kgbody weight BME treatment orally for one month with prior use of 15mg/kg b.w of MPTP for two weeks. After this, neuroprotective effect was studied through neurochemical analysis and in silico study. RESULTS: A significant recovery of spontaneous locomotor activity, antioxidant enzymatic activity was found. The increased levels of Dopamine (DA), 3-4 dihydroxy-phenyl acetic acid (DOPAC), Homovanillic acid (HVA) and decreased levels of lipid peroxides, conjugated dienes were evident as improvement in MPTP+BME group as compared to MPTP treated animals. In-silico study, revealed that BM phytoconstituents mainly bacosides, bacopasides and bacosaponins have capability to halt the breakdown of MPTP to MPP+ by inhibiting the enzyme Monoamineoxidases B. Further, BM phytochemicals bacosides, bacopasides and bacopasaponins showed interesting binding affinity with KEAP1 receptor protein and inhibiting the cullin3 mediated Nuclear factor like 2 (Nrf2) protein ubiquitination process and thereby up regulating the expression (via Nrf2) of antioxidant enzymes. CONCLUSION: Both in vivo and in silico studies indicate that BM phytochemical has ability to maintain DA concentrations in PD by virtue of DA synthesis induction and inhibition of DA degradation. This study may offer a therapeutic approach. KEYWORDS: Parkinson's disease, MPTP, Bacopa monnieri, Neurodegeneration, Oxidative stress.
ISSN:0970-1915