Effect of Different Fractions of methanolic Extract of Cinnamomumzeylanicumon Sheep Kidney Angiotensin Converting Enzyme: An In Vitro Study

INTRODUCTION: Angiotensin converting enzyme (ACE, EC 3.4.15.1), is a zinc metallopeptidase that cleaves Angiotensin I to Angiotensin II (a potent vasoconstrictor). It is most commonly associated with the regulation of blood pressure. Captopril, a known ACE inhibitor is used for treating hypertension...

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Published in:Indian journal of clinical biochemistry 2022-05, Vol.32 (S1), p.S176
Main Authors: Padmanabha, Udupa E.G, Ranjini, H.S, Bhat, Vinutha R
Format: Article
Language:English
Subjects:
Acetates
Angiotensin converting enzyme
Captopril
Esters
Health aspects
Hypertension
Laws, regulations and rules
Medicinal plants
Medicine, Botanic
Medicine, Herbal
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Summary:INTRODUCTION: Angiotensin converting enzyme (ACE, EC 3.4.15.1), is a zinc metallopeptidase that cleaves Angiotensin I to Angiotensin II (a potent vasoconstrictor). It is most commonly associated with the regulation of blood pressure. Captopril, a known ACE inhibitor is used for treating hypertension, which is well tolerated with certain side effects. Medicinal plant extracts are in use to treat many diseases and can be a good alternative for therapeutic outcomes because of their wellness, effectiveness and quality. AIM: The present study was aimed to estimate the effect of angiotensin converting enzyme (ACE) activity in presence of different fractions of methanolic extract of Cinnamomumzeylanicumon kidney ACE. METHOD: ACE activity of kidney tissue was measured with Hippuryl-Histidyl-Leucine (HHL) as substrate and the hippuric acid released was measured spectrophotometrically at 228nm. Similarly, ACE activity was measured in the presence of captopril and with different fractions (Petroleum ether, ethyl acetate and aqueous) of methanolic extract of Cinnamomum zeylanicumin the enzyme assay. RESULTS: The linearity of ACE activity in kidney was established with HHL as substrate for an incubation period of 30 min at 37[degrees]C. ACE activity was confirmed with specific ACE inhibitor Captopril. ACE activity was determined in the presence of petroleum ether, ethyl acetate and aqueous fractions of methanolic extract of C. zeylanicum. Ethyl acetate fraction has significantly inhibited ACE activity by 17.98% where as petroleum ether and aqueous fractions increased ACE activity by 11.7% and 4.9% respectively. CONCLUSION: Ethyl acetate fraction of methanolic extract contained ACE inhibitor, whereas petroleum ether and aqueous fractions contained ACE stimulants. Further isolation & identification of the active components and in vivo studies of these fractions may be useful in the treatment of hypertension and hypotension or related disorders. KEY WORDS: Angiotensin converting enzyme, Hippuryl-Histidyl-Leucine, Cinnamomum zeylanicumin,
ISSN:0970-1915