Symphytum officinale enhances osteogenesis in human bone marrow-derived mesenchymal stem cells in vitro

Mesenchymal stem cells (MSCs) are multipotent stem cells capable of self-renewal and differentiation. Symphytum officinale (SO) is a medicinal plant and in homoeopathic literature, is believed to accelerate bone healing. We wanted to determine if homoeopathic doses of SO could enhance osteogenesis i...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.33 (S1), p.S76
Main Authors: Dey, D, Agrawal, S.K, Shrivastava, V, Bhattacharya, A, Jingar, P, Manhas, J, Garg, B, Ansari, M.T, Khurana, A, Sen, S
Format: Article
Language:English
Subjects:
Analysis
Bone marrow
Enzyme-linked immunosorbent assay
Medicinal plants
Phosphatases
Stem cells
Transplantation
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Summary:Mesenchymal stem cells (MSCs) are multipotent stem cells capable of self-renewal and differentiation. Symphytum officinale (SO) is a medicinal plant and in homoeopathic literature, is believed to accelerate bone healing. We wanted to determine if homoeopathic doses of SO could enhance osteogenesis in MSCs in vitro. Bone marrow samples were collected from patients undergoing bone grafting procedures (n = 15). MSCs were isolated, expanded and characterized by flow cytometry (CD90, CD105). Cytotoxicity of SO was evaluated by MTT assay. Osteogenic differentiation was induced in MSCs with b-glycerophosphate, ascorbic acid and dexamethasone over 2 weeks. Different doses of SO were added to the basal differentiation media and efficiency of MSCs differentiating into osteoblasts were measured by flow cytometry (Osteocalcin) and alkaline phosphatase activity (ELISA). Gene expression analysis for osteoblast markers (Runx2, Osteopontin and Osteocalcin) was done in differentiated osteoblasts using Q-PCR. Flow cytometry (CD90, CD105) detected MSCs isolated from bone marrow (93-98%). MTT assay showed that SO did not induce any cytotoxicity in MSCs (24 h). The efficiency of osteogenic differentiation (2 weeks) for different doses of Symphytum officinale was determined by flow cytometry (n = 10) for osteoblast marker, Osteocalcin, and most doses of Symphytum officinale were found to enhance osteogenesis. Interestingly, gene expression analysis for Runx2, Osteopontin, Osteocalcin and alkaline phosphatase activity also showed increase in osteogenesis with Symphytum officinale, specially mother tincture. Our results indicate that osteogenesis is enhanced at a homoeopathic dose (mother tincture) of Symphytum officinale.
ISSN:0970-1915