Phytochemical Analysis and Dose Dependent Effect of Enicostemma Axillare on Breast Cancer Cell Line

Breast cancer is the second most common cancer in female population worldwide. For which various treatment like chemotherapy and radiotherapy are available. These lines of treatment do not decrease mortality but to a certain extent reduce morbidity. Medicinal plants have been used in traditional tre...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S95
Main Authors: Kamalam, R, Krishnamoorthy, G, Vijayaraghavan, R
Format: Article
Language:English
Subjects:
Analysis
Biological products
Breast cancer
Care and treatment
Health aspects
Hernia
Medicinal plants
Medicine, Botanic
Medicine, Herbal
Saponins
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Summary:Breast cancer is the second most common cancer in female population worldwide. For which various treatment like chemotherapy and radiotherapy are available. These lines of treatment do not decrease mortality but to a certain extent reduce morbidity. Medicinal plants have been used in traditional treatments for various human diseases since ancient times. Enicostemma axillare (E.axillare) found in India, exhibit anticancer effects against various cancers. It has been long since used in folk medicine to treat spectrum of disease like diabetes mellitus, rheumatism, abdominal ulcer, hernia, swelling, itching and insect poisoning. In these recent years, using advanced technologies, the therapeutic benefits of medicinal plants are generally traced to specific compounds; specifically the active constituents of the plants. Thus, this study was focused on the phytochemical analysis and standardization of E.axillare on MCF-7 cell line. The whole plant were collected from Chengalpet, Kanchipuram District, Tamil Nadu, India. The whole plant materials were shade dried and powdered. The plant extract was prepared by soxhlet apparatus using ethanol. With that extract phytochemical screening was done for alkaloids, flavonoids, steroids, carbohydrates, glycosides, amino acid, tannins, terpenoids and saponins. Standardization of E.axillare was done by MTT on MCF-9 cell line. Statistical analyses were conducted by one-way ANOVA in (SPSS) software version 16.0. The phytochemical analysis of E.axillare extract shows the presence of alkaloids, flavonoids, carbohydrates, glycosides, amino acid, tannins, terpenoids and saponins. MTT results that the IC50 value for E.axillare is 12.5 [micro]g/ml. The present study revealed that ethanol extract of E.axillare was rich in phytochemical constituents. Standardization of the drug may helpful to develop molecular studies, which might be of great contribution for the society. Thus, E.Axillare could be a potential natural therapeutic agent in future.
ISSN:0970-1915