Evaluation of Performance and Application of Two Nucleic acid Extraction Methods for Quantification of Plasma Epstein-Barr Virus DNA

To evaluate and compare the analytical performances and clinical application values of two nucleic acid extraction methods for the detection of plasma EBV-DNA. Nucleic acid was extracted parallel by silicon membrane centrifugal column method or automatic magnetic bead adsorption method, and EBV-DNA...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S147
Main Authors: Chen, Y, Zheng, Y.H, Chen, Y,S, Xiao, Z.Z
Format: Article
Language:English
Subjects:
Adsorption
Comparative analysis
DNA
Epstein-Barr virus
Genetic research
Methods
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Summary:To evaluate and compare the analytical performances and clinical application values of two nucleic acid extraction methods for the detection of plasma EBV-DNA. Nucleic acid was extracted parallel by silicon membrane centrifugal column method or automatic magnetic bead adsorption method, and EBV-DNA was determined by real-time fluorescence quantitative PCR. Using the third-party fixed value reference material, the detection performance of the two methods was compared, and the plasma of 100 patients with NPC and 100 healthy subjects were measured to evaluate the clinical value of the two methods. The accuracy and imprecision of the two methods for the extraction and detection of EBV-DNA met the requirements, and the results of clinical samples were linearly correlated. However, the repeatability of magnetic bead method was smaller and more stable than that of centrifugal column method (all < 7%), the minimum detection limit (about 3.334 * 101 IU/ml) was slightly more sensitive than that of centrifugal column method (4.159 * 101 IU/ml), and the positive rate and average viral load of NPC samples (95%, 8.342 * 103 IU/ml) were significantly higher than those of centrifugal column method (84%, 4.707 * 103 IU/ml) (P < 0.05). The automatic magnetic bead adsorption method for the extraction and detection of plasma EBV-DNA can achieve better detection performance and has higher clinical application value.
ISSN:0970-1915