Anti-Hyperlipidaemic and Antioxidant Activity of Ethyl Acetate Fractions of Khaya Senegalensis Stem Bark on Diet Induced Hyperlipidaemic Rats

Khaya senegalensis A. Juss (Meliaceae), is regarded as the most popular medicinal meliaceous plant family. The present study was conducted to evaluate anti-hyperlipidaemic and antioxidant potentials of column chromatography fractions from ethyl acetate extract of Khayasenegalensisstem bark. The chro...

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Published in:Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S179
Main Authors: Muhammad, I.U, Gadaka M.A, Alhassan, A.J, Wudil, A.M, Imam, A.A, Mohammed, A, Abdulmumin, Y
Format: Article
Language:English
Subjects:
Medicine, Botanic
Medicine, Herbal
Organic compounds
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Summary:Khaya senegalensis A. Juss (Meliaceae), is regarded as the most popular medicinal meliaceous plant family. The present study was conducted to evaluate anti-hyperlipidaemic and antioxidant potentials of column chromatography fractions from ethyl acetate extract of Khayasenegalensisstem bark. The chromatography was carried out using Silica gel [(60-120 mesh) (500g)] in slurry of n-hexane. The column was eluted using solvents and solvent mixtures of increasing polarity. The fractions obtained were monitored using analytical TLC technique on different solvent system, fractions with similar Rf value were pooled together and a total of seven fractions (FI-FVII) were obtained. Fifty rats (50) were grouped into ten groups of five rats each, rats from groups 111-X were induced with hyperlipidaemia using high fat diet. Group I served as Normal control, Group II served as hyperlipidaemic control, Group IK were administered with standard drug (Atorvastatin: 10mg/kg body weight) while Group IV-X were administered with 50mg/kg body weight of fraction I, II, III, IV, V, VI, and VII respectively. At the end of the experimental period (two weeks), the rats were euthanized and blood samples collected into a labelled centrifuge tube, centrifuged and serum obtained was used for analysis of Lipid profile, Antioxidant enzymes, Lactate dehydrogenase, Creatine kinase and HMG CoA reductase activities. A significant (p
ISSN:0970-1915