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Association between Vitamin B12 Metabolism Related Indicators and Polymorphism of TCN-2 Gene in Postpartum Women with Postpartum Depression

Vitamin B12 deficiency has been linked to the susceptibility of developing depression. Transcobalamin-2(TCN-2), is a glycoprotein essential for the intracellular transport of vitamin B12. A functional polymorphism in TCN-2 gene (rs1801198; G776C) influence the binding capacity of TCN-2 and may lead...

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Published in:Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S202
Main Authors: Dhiman, P, Rajendiran, S, Pillai, R, Bharadwaj, B, Veena, P, Premkumar, N
Format: Article
Language:English
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Summary:Vitamin B12 deficiency has been linked to the susceptibility of developing depression. Transcobalamin-2(TCN-2), is a glycoprotein essential for the intracellular transport of vitamin B12. A functional polymorphism in TCN-2 gene (rs1801198; G776C) influence the binding capacity of TCN-2 and may lead to intracellular deficiency of vitamin B12. We observed the association of this polymorphism with postpartum depression, and its effect on the circulating markers of vitamin B12 deficiency. Women were screened at 6 weeks postpartum for the probability of depression by EPDS score, and a score of >10 was taken as a cut-off (n=434; 217 in each group). Plasma was used for the estimation of homocysteine, methyl malonic acid (MMA), S-adenosyl methionine (SAM) and holotranscobalamin by commercially available ELISA kits and whole blood was used for extraction of DNA, which was further genotyped by real time PCR using Taqman probes. Of all the markers of vitamin B12 deficiency, total vitamin B12 was significantly lower (p-value [less than or equal to] 0.001) and methylmalonic acid (MMA) was significantly higher in women with probable depression (p-value= 0.002). Plasma homocysteine levels although higher in depressed group, were not statistically significant (p-value=0.057). No difference was observed between the distribution of genotypes (p-value-0.619) and alleles (p-value-0.398) between women with and without probable depression. On comparing the effect of various genotypes on circulating levels of holotranscobalamin, homocysteine and MMA, we observed GG genotype to be associated with higher MMA levels in women with probable depression. In conclusion, Genotype GG of TCN-2(rs1801198) is associated with higher MMA levels in women with probable depression, indicating decreased availability and cellular deficiency of vitamin B12.
ISSN:0970-1915