In Vitro Hemocompatibility and Genotoxicity Evaluation of Dual-Labeled [[sup.99m]Tc]Tc-FITC-Silk Fibroin Nanoparticles for Biomedical Applications

Nuclear imaging is a highly sensitive and noninvasive imaging technique that has become essential for medical diagnosis. The use of radiolabeled nanomaterials capable of acting as imaging probes has shown rapid development in recent years as a powerful, highly sensitive, and noninvasive tool. In add...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2023-02, Vol.16 (2)
Main Authors: Asensio Ruiz, María Alejandra, Alonso García, Ángela, Bravo-Ferrer Moreno, María de la Luz, Cebreiros-López, Iria, Noguera-Velasco, José Antonio, Lozano-Pérez, Antonio Abel, Martínez Martínez, Teresa
Format: Article
Language:English
Subjects:
Chemical properties
Diagnosis, Radioscopic
Health aspects
Methods
Nanoparticles
Radioactive tracers
Structure
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nuclear imaging is a highly sensitive and noninvasive imaging technique that has become essential for medical diagnosis. The use of radiolabeled nanomaterials capable of acting as imaging probes has shown rapid development in recent years as a powerful, highly sensitive, and noninvasive tool. In addition, quantitative single-photon emission computed tomography (SPECT) images performed by incorporating radioisotopes into nanoparticles (NPs) might improve the evaluation and the validation of potential clinical treatments. In this work, we present a direct method for [[sup.99m] Tc]Tc-radiolabeling of FITC-tagged silk fibroin nanoparticles (SFN). NPs were characterized by means of dynamic light scattering and scanning electron microscopy. In vitro studies were carried out, including the evaluation of stability in biological media and the evaluation of hemocompatibility and genotoxicity using the cytokinesis block micronucleus (CBMN) assay. The radiolabeling method was reproducible and robust with high radiolabeling efficiency (∼95%) and high stability in biological media. Hydrodynamic properties of the radiolabeled NPs remain stable after dual labeling. The interaction of SFN with blood elicits a mild host response, as expected. Furthermore, CBMN assay did not show genotoxicity induced by [[sup.99m] Tc]Tc-FITC-SFN under the described conditions. In conclusion, a feasible and robust dual-labeling method has been developed whose applicability has been demonstrated in vitro, showing its value for further investigations of silk fibroin NPs biodistribution in vivo.
ISSN:1424-8247
1424-8247
DOI:10.3390/ph16020248