Qualitative Screening of Yeast Biodiversity for Hydrolytic Enzymes Isolated from the Gastrointestinal Tract of a Coprophage “IGymnopleurus sturmi/I” and Dung of Ruminants

• Published in: Fermentation (Basel) 2022-11, Vol.8 (12)
• Main Authors: Hanane, Touijer, Najoua, Benchemsi, Salsabil, Hamdi, Abdellatif, Janati Idrissi, Dalila, Bousta, Ahmad, Irfan, BukharI, Sayyad Ali Raza, Irfan, Muhammad, Chen, Lijing, Hicham, Bekkari
• Format: Article
• Language: English
• Subjects: Beetles; Physiological aspects
• ISSN: 2311-5637; 2311-5637
• DOI: 10.3390/fermentation8120692

In this study, thirty yeast strains isolated from the gut of coprophagous “Gymnopleurus sturmi” and twenty-four from the dung of ruminants were shown to be producers of cellulases. Cellulolytic yeast isolates could also produce other hydrolytic enzymes such as pectinase, lipase, β-glucosidase, catalase, inulinase, urease, gelatinase, and protease. The oroduction of amylase was present in only one isolate of dung of ruminants. On the other hand, the production of tannase was absent in these isolates. All the yeasts isolated from two sources could utilize various carbon sources, including sorbitol, sucrose, and raffinose, and withstand high concentrations of glucose (300 g/L), salt (100 g/L), and exogenous ethanol. They could grow in a wide pH range of 3 to 11. The growth was stable up to a temperature of 40 °C for isolates from the gut of coprophage and 37 °C for the yeast from the dung of ruminants. These activities and growing conditions were similar to the diet of coprophagous insects and the composition of ruminant manure, likely because the adaptation and distribution of these microorganisms depend on the phenology and trophic preferences of these insects.