False Positive Results of Phosphatidylethanol : The Influence of Alcohol Vapors

The role of phosphatidylethanol (PEth) as an alcohol consumption marker is increasing in clinical and forensic medicine. During the COVID-19 pandemic, the role of hygiene increased, and it became common practice to use disinfectants almost everywhere. This paper highlights a possible source of false...

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Bibliographic Details
Published in:Separations 2022-09, Vol.9 (9)
Main Authors: Bashilov, Anton, Osipenko, Sergey, Ikonnikova, Karolina, Kovaleva, Oxana, Izotov, Boris, Nikolaev, Evgeny, Kostyukevich, Yury
Format: Article
Language:English
Subjects:
Biological markers
Blood
Chemical properties
Health aspects
Measurement
Medical examination
Phospholipids
Vapors
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Summary:The role of phosphatidylethanol (PEth) as an alcohol consumption marker is increasing in clinical and forensic medicine. During the COVID-19 pandemic, the role of hygiene increased, and it became common practice to use disinfectants almost everywhere. This paper highlights a possible source of false positive results (by the vapors of alcohols during the blood spot drying process) in dried blood spots (DBS) by LC-MS/MS quantitation of PEth. To achieve this, the PEth quantitation method was validated according to FDA guidelines. Additionally, the synthesis of phosphatidyl derivatives by phospholipase D (PLD) in the presence of methanol and 2-propanol vapors during the DBS process was determined. Each PEth-negative sample from a healthy male patient incubated in the presence of ethanol vapor becomes PEth-positive. After 4 h of DBS drying, teetotalers become “moderate drinkers”. It is necessary to avoid using alcohol-containing disinfectants in treatment rooms, where DBS is sampled.
ISSN:2297-8739
2297-8739
DOI:10.3390/separations9090250