Genomic and Phenotypic Characterization of ICutibacterium acnes/I Bacteriophages Isolated from Acne Patients

Cutibacteriumacnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 pha...

Full description

Saved in:
Bibliographic Details
Published in:Antibiotics (Basel) 2022-08, Vol.11 (8)
Main Authors: Kim, Shukho, Song, Hyesoon, Jin, Jong Sook, Lee, Weon Ju, Kim, Jungmin
Format: Article
Language:English
Subjects:
Infection
Skin
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cutibacteriumacnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT[sup.2] buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics11081041