Antimicrobial Resistance Challenged with Platinum-Thione in ICampylobacter jejuni/I

• Published in: Antibiotics (Basel) 2022-11, Vol.11 (11)
• Main Authors: Lacerda, Meiry Leandra de, Rossi, Daise Aparecida, Lourenzatto, Eduarda Cristina Alves, Takeuchi, Micaela Guidotti, Souza, Wesley Almeida, Silva, Raphael Tristão Cruvinel, Julio, Luma Gonçalves, Guerra, Wendell, Melo, Roberta Torres de
• Format: Article
• Language: English
• Subjects: Analysis; Banks (Finance); Drug resistance in microorganisms; Epidemiology; International economic relations; Palladium; Poultry industry
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics11111645

What are the main findings? Two new complexes containing mixed ligands were prepared and characterized; NMR data indicated that two thiolate ligands coordinate a metal ion via the sulphur atom after deprotonation. What is the implication of the main finding? The platinum complex was active against three strains of Campylobacter jejuni; A synergistic effect was observed when the Pt (II) complex was combined with ciprofloxacin. This work describes the synthesis and characterization of two metal complexes of the type [M(L[sub.1])[sub.2](phen)], where M = Pt[sup.2+] (complex I) or Pd[sup.2+] (complex II), L[sub.1] = 5-amino-1,3,4-thiadiazole-2(3H)-thiolate and phen = 1,10-phenanthroline. The in vitro antibacterial activity of these complexes was investigated in isolation and synergistically with ciprofloxacin (CIP) and erythromycin (ERY) in three strains of Campylobacter jejuni (MIC = 32 mg/L for CIP and ERY), selected from a bank of 235 strains representative of three poultry exporting states of the country (A, B and C), previously analyzed for epidemiology and resistance to CIP and ERY. A total of 53/235 (22.55%) strains showed co-resistance to CIP and ERY. Isolated resistance to CIP was higher than to ERY. Epidemiological analysis showed that resistance to CIP was more evident in state B (p < 0.0001), as well as a higher susceptibility to ERY in state C (p = 0.0028). Co-resistance was expressive in state A and in the spring and fall seasons. The evaluation of I alone and in synergy with CIP and ERY found values up to 0.25 mg/L not significant for ERY. Complex II did not show an antimicrobial effect on the three strains of tested C. jejuni. The effect provided by complex I represents a promising alternative for control of resistant strains of C. jejuni.