Synthesis and In Vitro Testing of YVO[sub.4]:Eu[sup.3+]@silica-NH-GDA-IgG Bio-Nano Complexes for Labelling MCF-7 Breast Cancer Cells

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-12, Vol.28 (1)
• Main Authors: Huong, Tran Thu, Vinh, Le Thi, Khuyen, Hoang Thi, Tuyen, Le Dac, Van, Nguyen Duc, Thao, Do Thi, Phuong, Ha Thi
• Format: Article
• Language: English
• Subjects: Analysis; Breast cancer; Cancer cells; Care and treatment; Diagnosis; Dosage and administration; Health aspects; Immunoglobulin G; Nanoparticles
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28010280

We present a visual tool and facile method to detect MCF-7 breast cancer cells by using YVO[sub.4] :Eu[sup.3+] @silica-NH-GDA-IgG bio-nanocomplexes. To obtain these complexes, YVO[sub.4] :Eu[sup.3+] nanoparticles with a uniform size of 10–25 nm have been prepared firstly by the hydrothermal process, followed by surface functionalization to be bio-compatible and conjugated with cancer cells. The YVO[sub.4] :Eu[sup.3+] @silica-NH-GDA-IgG nanoparticles exhibited an enhanced red emission at 618 nm under an excitation wavelength of 355 nm and were strongly coupled with MCF-7 breast cancer cells via biological conjugation. These bio-nanocomplexes showed a superior sensitiveness for MCF-7 cancer cell labelling with a detection percentage as high as 82%, while no HEK-293A healthy cells were probed under the same conditions of in vitro experiments. In addition, the detection percentage of MCF-7 breast cancer cells increased significantly via the functionalization and conjugation of YVO[sub.4] :Eu[sup.3+] nanoparticles. The experimental results demonstrated that the YVO[sub.4] :Eu[sup.3+] @silica-NH-GDA-IgG bio-nanocomplexes can be used as a promising labelling agent for biomedical imaging and diagnostics.