Effect of Plasma-Rich Growth Factors on Sperm Parameters in Teratozoospermic Samples

Background: A direct correlation exists between teratozoospermia and oxidative stress, which causes poor assisted reproductive techniques (ART) outcomes. This study aimed to investigate the effect of plasma-rich growth factors (PRGF) incubation on the quality of teratozoospermic samples. Methods: Se...

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Bibliographic Details
Published in:Iranian journal of medical sciences 2023-01, Vol.48 (S1), p.61
Main Authors: Nafchi, Hanieh Ghasemian, Azizi, Yaser, Halvaei, Iman, Amjadi, Fatemeh-Sadat
Format: Article
Language:English
Subjects:
Analysis
Chromatin
Spermatozoa
Online Access:Get full text
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Summary:Background: A direct correlation exists between teratozoospermia and oxidative stress, which causes poor assisted reproductive techniques (ART) outcomes. This study aimed to investigate the effect of plasma-rich growth factors (PRGF) incubation on the quality of teratozoospermic samples. Methods: Semen samples were collected from 25 teratozoospermic men attending clinics of Gandhi and Akbarabadi hospitals. After density gradient centrifugation, the spermatozoa culture medium was supplemented with different concentrations of PRGF (0%, 1%, 5%, and 10%). Sperm motility, viability (eosin-nigrosin), morphology (Papanicolaou), DNA fragmentation index (sperm chromatin dispersion test), mitochondrial membrane potential (JC-1 staining by flow cytometry), and lipid peroxidation (malondialdehyde) were measured and analyzed before and after one hour incubation. Results: Our results showed that after one-hour incubation, the addition of 1% PRGF improved sperm progressive motility (47.72[+ or -]13.76) compared to the control group (17.36[+ or -]8.50) (P
ISSN:0253-0716