The Effect of Sildenafil Citrate and Alpha-Lipoic Acid on the Sperm Biochemical Parameters during Cryopreservation in the Asthenozoospermic Infertile Men

Background: Cryopreservation is a common method for assisted reproductive technology that causes oxidative stress on sperm, which is the main reason for sperm disruption. Cryoprotective media are commonly supplemented with antioxidants that improve sperm quality post-thawing. This study investigated...

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Bibliographic Details
Published in:Iranian journal of medical sciences 2023-01, Vol.48 (S1), p.134
Main Authors: Kohzadi, Ronak, Mehranjani, Malek Soleimani, Shariatzadeh, Mohammadali, Cheraghi, Ebrahim
Format: Article
Language:English
Subjects:
Antioxidants
Enzyme-linked immunosorbent assay
Enzymes
Sildenafil
Spermatozoa
Superoxide
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Summary:Background: Cryopreservation is a common method for assisted reproductive technology that causes oxidative stress on sperm, which is the main reason for sperm disruption. Cryoprotective media are commonly supplemented with antioxidants that improve sperm quality post-thawing. This study investigated to assess the effects of Sildenafil Citrate (SC) and Alpha-Lipoic Acid (ALA) supplementation on oxidative stress during cryopreservation in asthenozoospermic infertile men. Methods: 30 semen samples were collected from the asthenozoospermic patients. Each semen sample was divided into five groups: Control (fresh), Freeze (treated with cryo-protectant alone), Freeze+SC (treated with cryo-protectant+0.67[micro]M SC), Freeze+ALA (treated with cryo-protectant+0.5mM ALA), and Freeze+SC+ALA. In each sample, sperm Tumor necrosis factor alpha (TNF-[alpha]), Malondialdehyde (MDA) level, and antioxidant enzymes, including Catalase (CAT), Glutathione (GSH), and Superoxide dismutase (SOD) levels were evaluated by Enzyme-linked immunosorbent assay (ELISA). Results: Our results showed that the freeze group had a significant reduction in antioxidant enzyme levels and a significant increase in sperm TNF-[alpha], and MDA levels compared to the control group (P
ISSN:0253-0716